2026-04-19T15:27:22Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/157132024-09-27T09:57:03Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Mashinchian, Omid author Hong, Xiaotong author Michaud, Joris author Migliavacca, Eugenia author Lefebvre, Gregory author Boss, Christophe author De Franceschi, Filippo author Le Moal, Emmeran author Collerette-Tremblay, Jasmin author Isern, Joan author Metairon, Sylviane author Raymond, Frederic author Descombes, Patrick author Bouche, Nicolas author Muñoz-Cánoves, Pura author Feige, Jerome N author Bentzinger, C Florian author 2022-03-04 Sustained exposure to a young systemic environment rejuvenates aged organisms and promotes cellular function. However, due to the intrinsic complexity of tissues it remains challenging to pinpoint niche-independent effects of circulating factors on specific cell populations. Here, we describe a method for the encapsulation of human and mouse skeletal muscle progenitors in diffusible polyethersulfone hollow fiber capsules that can be used to profile systemic aging in vivo independent of heterogeneous short-range tissue interactions. We observed that circulating long-range signaling factors in the old systemic environment lead to an activation of Myc and E2F transcription factors, induce senescence, and suppress myogenic differentiation. Importantly, in vitro profiling using young and old serum in 2D culture does not capture all pathways deregulated in encapsulated cells in aged mice. Thus, in vivo transcriptomic profiling using cell encapsulation allows for the characterization of effector pathways of systemic aging with unparalleled accuracy. Elife. 2022 Mar 4;11:e57393 http://hdl.handle.net/20.500.12105/15713 35245177 10.7554/eLife.57393 2050-084X eLife In vivo transcriptomic profiling using cell encapsulation identifies effector pathways of systemic aging.