2026-06-14T03:43:07Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/157102024-09-27T09:43:59Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Prummel, Karin D author Crowell, Helena L author Nieuwenhuize, Susan author Brombacher, Eline C author Daetwyler, Stephan author Soneson, Charlotte author Kresoja-Rakic, Jelena author Kocere, Agnese author Ronner, Manuel author Ernst, Alexander author Labbaf, Zahra author Clouthier, David E author Firulli, Anthony B author Sanchez-Iranzo, Hector author Naganathan, Sundar R author O'Rourke, Rebecca author Raz, Erez author Mercader, Nadia author Burger, Alexa author Felley-Bosco, Emanuela author Huisken, Jan author Robinson, Mark D author Mosimann, Christian author 2022-03-30 The mesothelium lines body cavities and surrounds internal organs, widely contributing to homeostasis and regeneration. Mesothelium disruptions cause visceral anomalies and mesothelioma tumors. Nonetheless, the embryonic emergence of mesothelia remains incompletely understood. Here, we track mesothelial origins in the lateral plate mesoderm (LPM) using zebrafish. Single-cell transcriptomics uncovers a post-gastrulation gene expression signature centered on hand2 in distinct LPM progenitor cells. We map mesothelial progenitors to lateral-most, hand2-expressing LPM and confirm conservation in mouse. Time-lapse imaging of zebrafish hand2 reporter embryos captures mesothelium formation including pericardium, visceral, and parietal peritoneum. We find primordial germ cells migrate with the forming mesothelium as ventral migration boundary. Functionally, hand2 loss disrupts mesothelium formation with reduced progenitor cells and perturbed migration. In mouse and human mesothelioma, we document expression of LPM-associated transcription factors including Hand2, suggesting re-initiation of a developmental program. Our data connects mesothelium development to Hand2, expanding our understanding of mesothelial pathologies. Nat Commun. 2022 Mar 30;13(1):1677 10.1038/s41467-022-29311-7 2041-1723 Nature communications 35354817 http://hdl.handle.net/20.500.12105/15710 Hand2 delineates mesothelium progenitors and is reactivated in mesothelioma.