2026-05-30T18:42:27Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/156702024-09-27T08:16:55Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Dosil, Sara G author Lopez-Cobo, Sheila author Rodriguez-Galan, Ana author Fernandez-Delgado, Irene author Ramirez-Huesca, Marta author Milan-Rois, Paula author Castellanos, Milagros author Somoza, Alvaro author Gómez, Manuel José author Reyburn, Hugh T author Vales-Gomez, Mar author Sánchez Madrid, Francisco author Fernandez-Messina, Lola author 2022-07-29 Natural killer (NK) cells recognize and kill target cells undergoing different types of stress. NK cells are also capable of modulating immune responses. In particular, they regulate T cell functions. Small RNA next-generation sequencing of resting and activated human NK cells and their secreted extracellular vesicles (EVs) led to the identification of a specific repertoire of NK-EV-associated microRNAs and their post-transcriptional modifications signature. Several microRNAs of NK-EVs, namely miR-10b-5p, miR-92a-3p, and miR-155-5p, specifically target molecules involved in Th1 responses. NK-EVs promote the downregulation of GATA3 mRNA in CD4+ T cells and subsequent TBX21 de-repression that leads to Th1 polarization and IFN-γ and IL-2 production. NK-EVs also have an effect on monocyte and moDCs (monocyte-derived dendritic cells) function, driving their activation and increased presentation and costimulatory functions. Nanoparticle-delivered NK-EV microRNAs partially recapitulate NK-EV effects in mice. Our results provide new insights on the immunomodulatory roles of NK-EVs that may help to improve their use as immunotherapeutic tools. Elife. 2022 Jul 29;11:e76319 10.7554/eLife.76319 2050-084X eLife 35904241 http://hdl.handle.net/20.500.12105/15670 Natural killer (NK) cell-derived extracellular-vesicle shuttled microRNAs control T cell responses.