2026-04-27T01:59:06Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/156602024-09-27T09:36:03Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Albericio, Guillermo
author
Aguilar, Susana
author
Torán, Jose Luis
author
Yañez, Rosa
author
López, Juan Antonio
author
Vazquez, Jesus
author
Mora, Carmen
author
Bernad, Antonio
funder
Unión Europea. Comisión Europea
funder
Ministerio de Ciencia e Innovación (España)
funder
Comunidad de Madrid (España)
funder
Instituto de Salud Carlos III
2023-03-16T15:15:16Z
2023-03-16T15:15:16Z
2022-01-07
Sci Rep. 2022 Jan 7;12(1):146
http://hdl.handle.net/20.500.12105/15660
34997006
10.1038/s41598-021-03956-8
2045-2322
Scientific reports
Clinical trials evaluating cardiac progenitor cells (CPC) demonstrated feasibility and safety, but no clear functional benefits. Therefore a deeper understanding of CPC biology is warranted to inform strategies capable to enhance their therapeutic potential. Here we have defined, using a label-free proteomic approach, the differential cytoplasmic and nuclear compartments of human CPC (hCPC). Global analysis of cytoplasmic repertoire in hCPC suggested an important hypoxia response capacity and active collagen metabolism. In addition, comparative analysis of the nuclear protein compartment identified a significant regulation of a small number of proteins in hCPC versus human mesenchymal stem cells (hMSC). Two proteins significantly upregulated in the hCPC nuclear compartment, IL1A and IMP3, showed also a parallel increase in mRNA expression in hCPC versus hMSC, and were studied further. IL1A, subjected to an important post-transcriptional regulation, was demonstrated to act as a dual-function cytokine with a plausible role in apoptosis regulation. The knockdown of the mRNA binding protein (IMP3) did not negatively impact hCPC viability, but reduced their proliferation and migration capacity. Analysis of a panel of putative candidate genes identified HMGA2 and PTPRF as IMP3 targets in hCPC. Therefore, they are potentially involved in hCPC proliferation/migration regulation.
eng
Comparative proteomic analysis of nuclear and cytoplasmic compartments in human cardiac progenitor cells.
journal article
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Comparative proteomic analysis Sci Rep 2022.pdf
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Comparative proteomic analysis Sci Rep 2022.pdf.txt