2026-04-28T10:37:05Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/156562024-09-27T08:06:36Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Crainiciuc, Georgiana author Palomino-Segura, Miguel author Molina-Moreno, Miguel author Sicilia, Jon author Aragones, David G author Li, Jackson Liang Yao author Madurga, Rodrigo author Adrover, Jose M author Aroca-Crevillen, Alejandra author Martin-Salamanca, Sandra author Del Valle, Alfonso Serrano author Castillo, Sandra D author Welch, Heidi C E author Soehnlein, Oliver author Graupera, Mariona author Sanchez-Cabo, Fatima author Zarbock, Alexander author Smithgall, Thomas E author Di Pilato, Mauro author Mempel, Thorsten R author Tharaux, Pierre-Louis author González, Santiago F author Ayuso-Sacido, Angel author Ng, Lai Guan author Calvo, Gabriel F author González-Díaz, Iván author Díaz-de-María, Fernando author Hidalgo, Andres author 2022-01 Transcriptional and proteomic profiling of individual cells have revolutionized interpretation of biological phenomena by providing cellular landscapes of healthy and diseased tissues1,2. These approaches, however, do not describe dynamic scenarios in which cells continuously change their biochemical properties and downstream 'behavioural' outputs3-5. Here we used 4D live imaging to record tens to hundreds of morpho-kinetic parameters describing the dynamics of individual leukocytes at sites of active inflammation. By analysing more than 100,000 reconstructions of cell shapes and tracks over time, we obtained behavioural descriptors of individual cells and used these high-dimensional datasets to build behavioural landscapes. These landscapes recognized leukocyte identities in the inflamed skin and trachea, and uncovered a continuum of neutrophil states inside blood vessels, including a large, sessile state that was embraced by the underlying endothelium and associated with pathogenic inflammation. Behavioural screening in 24 mouse mutants identified the kinase Fgr as a driver of this pathogenic state, and interference with Fgr protected mice from inflammatory injury. Thus, behavioural landscapes report distinct properties of dynamic environments at high cellular resolution. Nature. 2022 Jan;601(7893):415-421 http://hdl.handle.net/20.500.12105/15656 34987220 10.1038/s41586-021-04263-y 1476-4687 Nature Behavioural immune landscapes of inflammation.