2026-08-29T20:43:39Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/156532024-09-27T08:47:30Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Llorente, Vicente author Sanderson, Daniel author Martín-Gorgojo, Alejandro author Samaniego, Rafael author Desco, Manuel author Gómez-Gaviro, María Victoria author 2022-07-02 Cutaneous melanoma is one of the most aggressive and deadliest cancers in human beings due to its invasiveness and other factors. Histopathological analysis is crucial for a proper diagnosis. Optical tissue clearing is a novel field that allows 3D image acquisition of large-scale biological tissues. Optical clearing and immunolabeling for 3D fluorescence imaging has yet to be extensively applied to melanoma. In the present manuscript, we establish, for the first time, an optical clearing and immunostaining procedure for human melanoma and human cell line-derived melanoma xenograft models using the CUBIC (clear, unobstructed brain imaging cocktails) technique. We have successfully cleared the samples and achieved 3D volumetric visualization of the tumor microenvironment, vasculature, and cell populations. Biomedicines. 2022 Jul 2;10(7):1580 10.3390/biomedicines10071580 2227-9059 Biomedicines 35884885 http://hdl.handle.net/20.500.12105/15653 A 3D Analysis of Cleared Human Melanoma.