2026-07-25T12:19:45Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/156482024-09-27T08:12:39Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Tiana, María author Lopez-Jimenez, Elena author Sainz de Aja, Julio author Barral, Antonio author Victorino, Jesus author Badia-Careaga, Claudio author Rollan, Isabel author Rouco, Raquel author Santos, Elisa author Sanchez-Iranzo, Hector author Acemel, Rafael D author Torroja, Carlos author Adan, Javier author Andrés-León, Eduardo author Gomez-Skarmeta, Jose Luis author Giovinazzo, Giovanna author Sanchez-Cabo, Fatima author Manzanares, Miguel author 2022-07-15 Pluripotent cells are a transient population of the mammalian embryo dependent on transcription factors, such as OCT4 and NANOG, which maintain pluripotency while suppressing lineage specification. However, these factors are also expressed during early phases of differentiation, and their role in the transition from pluripotency to lineage specification is largely unknown. We found that pluripotency factors play a dual role in regulating key lineage specifiers, initially repressing their expression and later being required for their proper activation. We show that Oct4 is necessary for activation of HoxB genes during differentiation of embryonic stem cells and in the embryo. In addition, we show that the HoxB cluster is coordinately regulated by OCT4 binding sites located at the 3' end of the cluster. Our results show that core pluripotency factors are not limited to maintaining the precommitted epiblast but are also necessary for the proper deployment of subsequent developmental programs. Sci Adv. 2022 Jul 15;8(28):eabo3583 10.1126/sciadv.abo3583 2375-2548 Science advances 35857513 http://hdl.handle.net/20.500.12105/15648 Pluripotency factors regulate the onset of Hox cluster activation in the early embryo.