2026-04-19T15:10:06Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/156422024-09-27T08:10:57Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Herrero-Galán, Elías author Martínez-Martín, Inés author Sanchez-Gonzalez, Cristina author Vicente, Natalia author Bonzón-Kulichenko, Elena author Calvo, Enrique author Suay-Corredera, Carmen author Pricolo, Maria Rosaria author Fernández-Trasancos, Ángel author Velázquez-Carreras, Diana author Careaga, Claudio Badía author Abdellatif, Mahmoud author Sedej, Simon author Rainer, Peter P author Giganti, David author Pérez-Jiménez, Raúl author Vazquez, Jesus author Alegre-Cebollada, Jorge author 2022-06 Titin, as the main protein responsible for the passive stiffness of the sarcomere, plays a key role in diastolic function and is a determinant factor in the etiology of heart disease. Titin stiffness depends on unfolding and folding transitions of immunoglobulin-like (Ig) domains of the I-band, and recent studies have shown that oxidative modifications of cryptic cysteines belonging to these Ig domains modulate their mechanical properties in vitro. However, the relevance of this mode of titin mechanical modulation in vivo remains largely unknown. Here, we describe the high evolutionary conservation of titin mechanical cysteines and show that they are remarkably oxidized in murine cardiac tissue. Mass spectrometry analyses indicate a similar landscape of basal oxidation in murine and human myocardium. Monte Carlo simulations illustrate how disulfides and S-thiolations on these cysteines increase the dynamics of the protein at physiological forces, while enabling load- and isoform-dependent regulation of titin stiffness. Our results demonstrate the role of conserved cysteines in the modulation of titin mechanical properties in vivo and point to potential redox-based pathomechanisms in heart disease. Redox Biol. 2022 Jun;52:102306 http://hdl.handle.net/20.500.12105/15642 35367810 10.1016/j.redox.2022.102306 2213-2317 Redox biology Basal oxidation of conserved cysteines modulates cardiac titin stiffness and dynamics.