2026-04-22T17:52:02Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/156382024-09-27T09:46:15Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Marques, Ines J author Ernst, Alexander author Arora, Prateek author Vianin, Andrej author Hetke, Tanja author Sanz-Morejón, Andrés author Naumann, Uta author Odriozola, Adolfo author Langa, Xavier author Andres-Delgado, Laura author Zuber, Benoît author Torroja, Carlos author Osterwalder, Marco author Simões, Filipa C author Englert, Christoph author Mercader, Nadia author 2022-03-15 During development, the heart grows by addition of progenitor cells to the poles of the primordial heart tube. In the zebrafish, Wilms tumor 1 transcription factor a (wt1a) and b (wt1b) genes are expressed in the pericardium, at the venous pole of the heart. From this pericardial layer, the proepicardium emerges. Proepicardial cells are subsequently transferred to the myocardial surface and form the epicardium, covering the myocardium. We found that while wt1a and wt1b expression is maintained in proepicardial cells, it is downregulated in pericardial cells that contributes cardiomyocytes to the developing heart. Sustained wt1b expression in cardiomyocytes reduced chromatin accessibility of specific genomic loci. Strikingly, a subset of wt1a- and wt1b-expressing cardiomyocytes changed their cell-adhesion properties, delaminated from the myocardium and upregulated epicardial gene expression. Thus, wt1a and wt1b act as a break for cardiomyocyte differentiation, and ectopic wt1a and wt1b expression in cardiomyocytes can lead to their transdifferentiation into epicardial-like cells. Development. 2022 Mar 15;149(6):dev200375 http://hdl.handle.net/20.500.12105/15638 35312773 10.1242/dev.200375 1477-9129 Development (Cambridge, England) Wt1 transcription factor impairs cardiomyocyte specification and drives a phenotypic switch from myocardium to epicardium.