2026-06-14T03:40:17Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/155522025-04-25T08:32:52Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19616
Repisalud
author
Abarrategi, Ander
author
Perez-Tavarez, Raquel
author
Rodriguez-Milla, Miguel A
author
Cubillo, Isabel
author
Mulero, Francisca
author
Alfranca, Arantzazu
author
López-Lacomba, José Luís
author
Garcia-Castro, Javier
funder
Unión Europea. Comisión Europea. 7 Programa Marco
funder
Instituto de Salud Carlos III
funder
Comunidad de Madrid (España)
funder
Ministerio de Ciencia e Innovación (España)
funder
Fundación Mapfre
2023-02-28T14:11:33Z
2023-02-28T14:11:33Z
2013-12
Stem Cell Rev Rep. 2013 Dec;9(6):833-46.
10.1007/s12015-013-9464-1
2629-3277
Stem cell reviews and reports
23934266
http://hdl.handle.net/20.500.12105/15552
Clinical interest on human mesenchymal progenitor cells (hMPC) relies on their potential applicability in cell-based therapies. An in vitro characterization is usually performed in order to define MPC potency. However, in vitro predictions not always correlate with in vivo results and thus there is no consensus in how to really assess cell potency. Our goal was to provide an in vivo testing method to define cell behavior before therapeutic usage, especially for bone tissue engineering applications. In this context, we wondered whether bone marrow stromal cells (hBMSC) would proceed in an osteogenic microenvironment. Based on previous approaches, we developed a fibrin/ceramic/BMP-2/hBMSCs compound. We implanted the compound during only 2 weeks in NOD-SCID mice, either orthotopically to assess its osteoinductive property or subcutaneously to analyze its adequacy as a cell potency testing method. Using fluorescent cell labeling and immunohistochemistry techniques, we could ascertain cell differentiation to bone, bone marrow, cartilage, adipocyte and fibrous tissue. We observed differences in cell potential among different batches of hBMSCs, which did not strictly correlate with in vitro analyses. Our data indicate that the method we have developed is reliable, rapid and reproducible to define cell potency, and may be useful for testing cells destined to bone tissue engineering purposes. Additionally, results obtained with hMPCs from other sources indicate that our method is suitable for testing any potentially implantable mesenchymal cell. Finally, we propose that this model could successfully be employed for bone marrow niche and bone tumor studies.
eng
Mesenchymal cells
Ceramics
BMP-2
Animal mode
In vivo ectopic implantation model to assess human mesenchymal progenitor cell potential
research article
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InVivoEctopicImplantationModel_2013.pdf
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https://repisalud.isciii.es/bitstreams/64504a0c-79ad-4771-bcb6-f0bd128a79fd/download
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text/plain
InVivoEctopicImplantationModel_2013.pdf.txt