2026-05-20T23:22:38Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/155182024-11-28T18:45:20Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16963col_20.500.12105_16977
Repisalud
author
Rodrigo-Muñoz, José M
author
Gil-Martínez, Marta
author
Lorente-Sorolla, Clara
author
Sastre, Beatriz
author
García-García, María Luz
author
Calvo, Cristina
author
Casas Flecha, Inmaculada
author
Del Pozo, Victoria
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Alfonso X el Sabio University (España)
funder
Fundación Banco Santander
funder
Centro de Investigación Biomédica en Red - CIBERES (Enfermedades Respiratorias)
2023-02-21T09:16:51Z
2023-02-21T09:16:51Z
2022-09-02
Cells. 2022 Sep 2;11(17):2746.
10.3390/cells11172746
2073-4409
Cells
36078154
http://hdl.handle.net/20.500.12105/15518
Respiratory diseases such as bronchiolitis, and those with wheezing episodes, are highly important during infancy due to their potential chronicity. Immune response dysregulation is critical in perpetuating lung damage. Epigenetic modifications including microRNA (miRNA) post-transcriptional regulation are among the factors involved in alleviating inflammation. We evaluated the expression of miR-146a-5p, a previously described negative regulator of immunity, in infants with respiratory diseases, in order to study epigenetic regulation of the immune response. Nasopharyngeal aspirate (NPA) was obtained from infants with bronchiolitis (ongoing and post-disease) or with wheezing episodes in addition to healthy controls. Virus presence was determined by nested PCR, while miRNA and gene expression were studied in cells from NPAs using qPCR. Healthy small airway epithelial cells (SAECs) were used as an in vitro model. We observe a reduction in miR-146a-5p expression in infants with either of the two diseases compared to controls, suggesting the potential of this miRNA as a disease biomarker. Post-bronchiolitis, miR-146a-5p expression increases, though without reaching levels of healthy controls. MiR-146a-5p expression correlates inversely with the immune-related gene PTGS2, while its expression correlates directly with TSLP. When heathy donor SAECs are stimulated by poly:IC, we observe an increase in miR-146a-5p, with wounds having a synergistic effect. In conclusion, infants with respiratory diseases present reduced miR-146a-5p expression, possibly affecting immune dysregulation.
eng
Infant respiratory diseases
microRNAs
Immune regulation
Reduced miR-146a-5p Is a Biomarker of Infant Respiratory Diseases Contributing to Immune Dysregulation in Small Airway Epithelial Cells
journal article
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ReducedmiR-146a-5pBiomarkerInfant_2022.pdf
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ReducedmiR-146a-5pBiomarkerInfant_2022.pdf.txt