2026-06-14T03:51:18Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/154922024-09-27T22:20:46Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Garcia-Barreno, Blanca
author
Delgado-Romero, Teresa
author
Benito, Sonia
author
Casas Flecha, Inmaculada
author
Pozo Sanchez, Francisco
author
Melero, Jose Antonio
funder
Instituto de Salud Carlos III
funder
Ministerio de Asuntos Económicos y Transformación Digital (España)
2023-02-10T14:05:16Z
2023-02-10T14:05:16Z
2014-10
J Gen Virol. 2014 Oct;95(Pt 10):2140-2145.
10.1099/vir.0.067413-0
1465-2099
The Journal of general virology
25000959
http://hdl.handle.net/20.500.12105/15492
Alternative methods to the standard haemagglutination inhibition (HI) and neutralization tests to probe the antigenic properties of the influenza virus haemagglutinin (HA) were developed in this study. Vaccinia virus recombinants expressing reference HAs were used to immunize rabbits from which polyclonal antibodies were obtained. These antibodies were subtype specific but showed limited intra-subtype strain specificity in ELISA. The discriminatory capacity of these antibodies was, however, markedly increased after adsorption to cells infected with heterologous influenza viruses, revealing antigenic differences that were otherwise undistinguishable by standard HI and neutralization tests. Furthermore, the unadsorbed antibodies could be used to select escape mutants of the reference strain, which after sequencing unveiled amino acid changes responsible of the noted antigenic differences. These procedures therefore provide alternative methods for the antigenic characterization of influenza HA and might be useful in studies of HA antigenic evolution.
eng
Exploring the antigenic relatedness of influenza virus haemagglutinins with strain-specific polyclonal antibodies
journal article
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https://repisalud.isciii.es/bitstreams/d99492b3-4fe2-45dd-833d-a1eecea73389/download
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ExploringAntigenicRelatednessInfluenzaVirus_2014.pdf
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https://repisalud.isciii.es/bitstreams/8c37dd1e-325f-4d4b-a0d2-6781ddf1b213/download
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text/plain
ExploringAntigenicRelatednessInfluenzaVirus_2014.pdf.txt