2026-04-29T19:34:53Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/154532024-11-28T16:31:27Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16938
Repisalud
author
Cañada-Garcia, Javier Enrique
author
Ramirez de Arellano, Eva
author
Jiménez-Orellana, Miguel
author
Viedma, Esther
author
Sánchez, Aida
author
Alhambra, Almudena
author
Villa, Jennifer
author
Delgado-Iribarren, Alberto
author
Bautista, Veronica
author
Lara Fuella, Noelia
author
García-Cobos, Silvia
author
Aracil, Belen
author
Cercenado, Emilia
author
Perez-Vazquez, Maria
author
Oteo-Iglesias, Jesus
funder
Instituto de Salud Carlos III
funder
Centro de Investigación Biomédica en Red - CIBERINFEC (Enfermedades Infecciosas)
funder
Ministerio de Ciencia e Innovación (España)
funder
Unión Europea
funder
Plan Nacional de I+D+i (España)
funder
RETICS-Investigación en Patología Infecciosa (REIPI-ISCIII) (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
2023-02-03T19:57:02Z
2023-02-03T19:57:02Z
2023-01-06
Antibiotics (Basel). 2023 Jan 6;12(1):107.
10.3390/antibiotics12010107
2079-6382
Antibiotics (Basel, Switzerland)
36671308
http://hdl.handle.net/20.500.12105/15453
During the COVID-19 pandemic, intensive care units (ICUs) operated at or above capacity, and the number of ICU patients coinfected by nosocomial microorganisms increased. Here, we characterize the population structure and resistance mechanisms of carbapenemase-producing Klebsiella pneumoniae (CP-Kpn) from COVID-19 ICU patients and compare them to pre-pandemic populations of CP-Kpn. We analyzed 84 CP-Kpn isolates obtained during the pandemic and 74 CP-Kpn isolates obtained during the pre-pandemic period (2019) by whole genome sequencing, core genome multilocus sequence typing, plasmid reconstruction, and antibiotic susceptibility tests. More CP-Kpn COVID-19 isolates produced OXA-48 (60/84, 71.4%) and VIM-1 (18/84, 21.4%) than KPC (8/84, 9.5%). Fewer pre-pandemic CP-Kpn isolates produced VIM-1 (7/74, 9.5%). Cefiderocol (97.3-100%) and plazomicin (97.5-100%) had the highest antibiotic activity against pandemic and pre-pandemic isolates. Sequence type 307 (ST307) was the most widely distributed ST in both groups. VIM-1-producing isolates belonging to ST307, ST17, ST321 and ST485, (STs infrequently associated to VIM-1) were detected during the COVID-19 period. Class 1 integron Int1-blaVIM-1-aac(6')-1b-dfrB1-aadAI-catB2-qacEΔ1/sul1, found on an IncL plasmid of approximately 70,000 bp, carried blaVIM-1 in ST307, ST17, ST485, and ST321 isolates. Thus, CP-Kpn populations from pandemic and pre-pandemic periods have similarities. However, VIM-1 isolates associated with atypical STs increased during the pandemic, which warrants additional monitoring and surveillance.
eng
Klebsiella pneumoniae
Carbapenemases
COVID-19
Antibiotic resistance
Intensive care units (ICUs)
Outbreaks
cgMLST
WGS
Carbapenemase-Producing Klebsiella pneumoniae in COVID-19 Intensive Care Patients: Identification of IncL-VIM-1 Plasmid in Previously Non-Predominant Sequence Types
journal article
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Carbapenemase-ProducingKlebsiellaPneumoniae_2023.pdf
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a7212b8ad318e3bea6765f6bd5feabcc
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Carbapenemase-ProducingKlebsiellaPneumoniae_2023.pdf.txt