2026-07-22T05:26:12Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/154522024-11-28T16:39:37Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16958col_20.500.12105_16963col_20.500.12105_16967col_20.500.12105_16970col_20.500.12105_16982col_20.500.12105_16985col_20.500.12105_16972col_20.500.12105_16988
Repisalud
author
Cañada-Garcia, Javier Enrique
author
Moure García, Zaira
author
Sola-Campoy, Pedro Juan
author
Delgado-Valverde, Mercedes
author
Cano, María E
author
Gijón, Desirèe
author
González, Mónica
author
Gracia-Ahufinger, Irene
author
Larrosa, Nieves
author
Mulet, Xavier
author
Pitart, Cristina
author
Rivera, Alba
author
Bou, Germán
author
Calvo, Jorge
author
Cantón, Rafael
author
González-López, Juan José
author
Martínez-Martínez, Luis
author
Navarro, Ferran
author
Oliver, Antonio
author
Palacios-Baena, Zaira R
author
Pascual, Álvaro
author
Ruiz-Carrascoso, Guillermo
author
Vila, Jordi
author
Aracil, Belen
author
Perez-Vazquez, Maria
author
Oteo-Iglesias, Jesus
author
GEMARA/GEIRAS-SEIMC/REIPI CARB-ES-19 Study Group
funder
Instituto de Salud Carlos III
funder
Plan Nacional de I+D+i (España)
funder
Ministerio de Economía y Competitividad (España)
funder
RETICS-Investigación en Patología Infecciosa (REIPI-ISCIII) (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Centro de Investigación Biomédica en Red - CIBERINFEC (Enfermedades Infecciosas)
funder
Unión Europea
2023-02-03T19:33:51Z
2023-02-03T19:33:51Z
2022-06-30
Front Microbiol. 2022 Jun 30;13:918362.
10.3389/fmicb.2022.918362
1664-302X
Frontiers in microbiology
35847090
http://hdl.handle.net/20.500.12105/15452
Objectives: CARB-ES-19 is a comprehensive, multicenter, nationwide study integrating whole-genome sequencing (WGS) in the surveillance of carbapenemase-producing K. pneumoniae (CP-Kpn) and E. coli (CP-Eco) to determine their incidence, geographical distribution, phylogeny, and resistance mechanisms in Spain. Methods: In total, 71 hospitals, representing all 50 Spanish provinces, collected the first 10 isolates per hospital (February to May 2019); CPE isolates were first identified according to EUCAST (meropenem MIC > 0.12 mg/L with immunochromatography, colorimetric tests, carbapenem inactivation, or carbapenem hydrolysis with MALDI-TOF). Prevalence and incidence were calculated according to population denominators. Antibiotic susceptibility testing was performed using the microdilution method (EUCAST). All 403 isolates collected were sequenced for high-resolution single-nucleotide polymorphism (SNP) typing, core genome multilocus sequence typing (cgMLST), and resistome analysis. Results: In total, 377 (93.5%) CP-Kpn and 26 (6.5%) CP-Eco isolates were collected from 62 (87.3%) hospitals in 46 (92%) provinces. CP-Kpn was more prevalent in the blood (5.8%, 50/853) than in the urine (1.4%, 201/14,464). The cumulative incidence for both CP-Kpn and CP-Eco was 0.05 per 100 admitted patients. The main carbapenemase genes identified in CP-Kpn were bla OXA-48 (263/377), bla KPC-3 (62/377), bla VIM-1 (28/377), and bla NDM-1 (12/377). All isolates were susceptible to at least two antibiotics. Interregional dissemination of eight high-risk CP-Kpn clones was detected, mainly ST307/OXA-48 (16.4%), ST11/OXA-48 (16.4%), and ST512-ST258/KPC (13.8%). ST512/KPC and ST15/OXA-48 were the most frequent bacteremia-causative clones. The average number of acquired resistance genes was higher in CP-Kpn (7.9) than in CP-Eco (5.5). Conclusion: This study serves as a first step toward WGS integration in the surveillance of carbapenemase-producing Enterobacterales in Spain. We detected important epidemiological changes, including increased CP-Kpn and CP-Eco prevalence and incidence compared to previous studies, wide interregional dissemination, and increased dissemination of high-risk clones, such as ST307/OXA-48 and ST512/KPC-3.
eng
CARB-ES-19 study
Carbapenemases
Whole genome sequencing
Klebsiella pneumoniae
High-risk clones
CARB-ES-19 Multicenter Study of Carbapenemase-Producing Klebsiella pneumoniae and Escherichia coli From All Spanish Provinces Reveals Interregional Spread of High-Risk Clones Such as ST307/OXA-48 and ST512/KPC-3.
journal article
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URL
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application/pdf
CARB-ES-19MulticenterStudyCarbapenemase-Producing_2022.pdf
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https://repisalud.isciii.es/bitstreams/fbbc4ace-55d2-4e29-ba3e-5675ff40cbef/download
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application/pdf
Corrigendum_CARB-ES-19MulticenterStudyCarbapenemase-Producing_2023.pdf
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CARB-ES-19MulticenterStudyCarbapenemase-Producing_2022.pdf.txt
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Corrigendum_CARB-ES-19MulticenterStudyCarbapenemase-Producing_2023.pdf.txt