2026-05-22T00:30:05Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/153982024-09-27T09:37:07Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Lolo, Fidel-Nicolás author Walani, Nikhil author Seemann, Eric author Zalvidea, Dobryna author Pavón, Dácil María author Cojoc, Gheorghe author Zamai, Moreno author Viaris de Lesegno, Christine author Martínez de Benito, Fernando author Sánchez-Álvarez, Miguel author Uriarte, Juan José author Echarri, Asier author Jiménez-Carretero, Daniel author Escolano, Joan-Carles author Sánchez, Susana A author Caiolfa, Valeria R author Navajas, Daniel author Trepat, Xavier author Guck, Jochen author Lamaze, Christophe author Roca-Cusachs, Pere author Kessels, Michael M author Qualmann, Britta author Arroyo, Marino author del Pozo, Miguel Angel author 2022-12-21 In response to different types and intensities of mechanical force, cells modulate their physical properties and adapt their plasma membrane (PM). Caveolae are PM nano-invaginations that contribute to mechanoadaptation, buffering tension changes. However, whether core caveolar proteins contribute to PM tension accommodation independently from the caveolar assembly is unknown. Here we provide experimental and computational evidence supporting that caveolin-1 confers deformability and mechanoprotection independently from caveolae, through modulation of PM curvature. Freeze-fracture electron microscopy reveals that caveolin-1 stabilizes non-caveolar invaginations-dolines-capable of responding to low-medium mechanical forces, impacting downstream mechanotransduction and conferring mechanoprotection to cells devoid of caveolae. Upon cavin-1/PTRF binding, doline size is restricted and membrane buffering is limited to relatively high forces, capable of flattening caveolae. Thus, caveolae and dolines constitute two distinct albeit complementary components of a buffering system that allows cells to adapt efficiently to a broad range of mechanical stimuli. Nat Cell Biol. 2022 Dec 21 10.1038/s41556-022-01034-3 1476-4679 Nature cell biology 36543981 http://hdl.handle.net/20.500.12105/15398 Caveolin-1 dolines form a distinct and rapid caveolae-independent mechanoadaptation system.