2026-06-14T03:47:11Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/152942025-04-29T12:54:15Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Martinez-Roman, Paula
author
Crespo-Bermejo, Celia
author
Valle-Millares, Daniel
author
Lara-Aguilar, Violeta
author
Arca de Lafuente, Sonia
author
Martín-Carbonero, Luz
author
Ryan, Pablo
author
de Los Santos, Ignacio
author
Lopez-Huertas, Maria Rosa
author
Palladino, Claudia
author
Muñoz-Muñoz, María
author
Fernandez-Rodriguez, Amanda
author
Coiras, Mayte
author
Briz, Veronica
author
Multidisciplinary HIV/Hepatitis Viral Coinfection Group (COVIHEP)
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Fundação para a Ciência e Tecnologia (Portugal)
funder
National Institutes of Health (Estados Unidos)
funder
Red de Investigación Cooperativa en Investigación en Sida (España)
2022-12-20T13:05:14Z
2022-12-20T13:05:14Z
2022-06-21
J Clin Med. 2022 Jun 21;11(13):3579.
10.3390/jcm11133579
2077-0383
Journal of Clinical Medicine
35806864
http://hdl.handle.net/20.500.12105/15294
Background: Although human immunodeficiency virus type 1 (HIV-1) reservoir size is very stable under antiretroviral therapy (ART), individuals exposed to the Hepatitis C virus (HCV) (chronically coinfected and spontaneous clarifiers) show an increase in HIV reservoir size and in spliced viral RNA, which could indicate that the viral protein regulator Tat is being more actively synthesized and, thus, could lead to a higher yield of new HIV. However, it is still unknown whether the effect of HCV elimination with direct-acting antivirals (DAAs) could modify the HIV reservoir and splicing. Methods: This longitudinal study (48 weeks' follow-up after sustained virological response) involves 22 HIV+-monoinfected individuals, 17 HIV+/HCV- spontaneous clarifiers, and 24 HIV+/HCV+ chronically infected subjects who eliminated HCV with DAAs (all of them aviremic, viral load < 50). Viral-spliced RNA transcripts and proviral DNA copies were quantified by qPCR. Paired samples were analyzed using a mixed generalized linear model. Results: A decrease in HIV proviral DNA was observed in HIV+/HCV- subjects, but no significant differences were found for the other study groups. An increased production of multiple spliced transcripts was found in HIV+ and HIV+/HCV+ individuals. Conclusions: We conclude that elimination of HCV by DAAs was unable to revert the consequences derived from chronic HCV infection for the reservoir size and viral splicing, which could indicate an increased risk of rapid HIV-reservoir reactivation. Moreover, spontaneous clarifiers showed a significant decrease in the HIV reservoir, likely due to an enhanced immune response in these individuals.
eng
DAAs
HIV reservoir
HIV/HCV
Coinfection
Dynamics
Spontaneous HCV clearance
Viral splicing
Dynamics of HIV Reservoir and HIV-1 Viral Splicing in HCV-Exposed Individuals after Elimination with DAAs or Spontaneous Clearance
research article
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