2026-06-14T03:54:14Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/152942025-04-29T12:54:15Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Martinez-Roman, Paula author Crespo-Bermejo, Celia author Valle-Millares, Daniel author Lara-Aguilar, Violeta author Arca de Lafuente, Sonia author Martín-Carbonero, Luz author Ryan, Pablo author de Los Santos, Ignacio author Lopez-Huertas, Maria Rosa author Palladino, Claudia author Muñoz-Muñoz, María author Fernandez-Rodriguez, Amanda author Coiras, Mayte author Briz, Veronica author Multidisciplinary HIV/Hepatitis Viral Coinfection Group (COVIHEP) author 2022-06-21 Background: Although human immunodeficiency virus type 1 (HIV-1) reservoir size is very stable under antiretroviral therapy (ART), individuals exposed to the Hepatitis C virus (HCV) (chronically coinfected and spontaneous clarifiers) show an increase in HIV reservoir size and in spliced viral RNA, which could indicate that the viral protein regulator Tat is being more actively synthesized and, thus, could lead to a higher yield of new HIV. However, it is still unknown whether the effect of HCV elimination with direct-acting antivirals (DAAs) could modify the HIV reservoir and splicing. Methods: This longitudinal study (48 weeks' follow-up after sustained virological response) involves 22 HIV+-monoinfected individuals, 17 HIV+/HCV- spontaneous clarifiers, and 24 HIV+/HCV+ chronically infected subjects who eliminated HCV with DAAs (all of them aviremic, viral load < 50). Viral-spliced RNA transcripts and proviral DNA copies were quantified by qPCR. Paired samples were analyzed using a mixed generalized linear model. Results: A decrease in HIV proviral DNA was observed in HIV+/HCV- subjects, but no significant differences were found for the other study groups. An increased production of multiple spliced transcripts was found in HIV+ and HIV+/HCV+ individuals. Conclusions: We conclude that elimination of HCV by DAAs was unable to revert the consequences derived from chronic HCV infection for the reservoir size and viral splicing, which could indicate an increased risk of rapid HIV-reservoir reactivation. Moreover, spontaneous clarifiers showed a significant decrease in the HIV reservoir, likely due to an enhanced immune response in these individuals. J Clin Med. 2022 Jun 21;11(13):3579. 10.3390/jcm11133579 2077-0383 Journal of Clinical Medicine 35806864 http://hdl.handle.net/20.500.12105/15294 DAAs HIV reservoir HIV/HCV Coinfection Dynamics Spontaneous HCV clearance Viral splicing Dynamics of HIV Reservoir and HIV-1 Viral Splicing in HCV-Exposed Individuals after Elimination with DAAs or Spontaneous Clearance