2026-06-14T03:38:32Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/152872025-06-09T15:06:47Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16961
Repisalud
author
Xufré, Cristina
author
González, Tanía
author
Leal, Lorna
author
Trubey, Charles M
author
Lifson, Jeffrey D
author
Gatell, José María
author
Alcamí, José
author
Climent, Núria
author
García, Felipe
author
Sánchez-Palomino, Sonsoles
funder
Ministerio de Economía (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Instituto de Salud Carlos III
funder
Government of Catalonia (España)
funder
Fundación La Caixa
funder
NIH - National Cancer Institute (NCI) (Estados Unidos)
funder
Red de Investigación Cooperativa en Investigación en Sida (España)
2022-12-20T07:10:28Z
2022-12-20T07:10:28Z
2022-04
Front Virol. 2022 Apr;2:869431.
10.3389/fviro.2022.869431
2673-818X
Frontiers in Virology
35967461
http://hdl.handle.net/20.500.12105/15287
We described a novel HIV autologous isolation method based in coculturing macrophages and CD4+T-cell-enriched fractions from peripheral blood collected from antiretroviral-treated (ART) HIV patients. This method allows the isolation of high viral titers of autologous viruses, over 1010HIV RNA copies/ml, and reduces the time required to produce necessary amounts for virus for use as antigens presented by monocyte-derived myeloid cells in HIV therapeutic vaccine approaches. By applying these high titer and autologous virus produced in the patient-derived cells, we intended to elicit a boost of the immunological system response in HIV therapeutic vaccines in clinical trials.
eng
CD4+ T cells
HIV isolation
HIV therapeutic vaccine
Autologous HIV immunogen
Coculture
Monocyte-derived macrophages (MDM)
Highly Efficient Autologous HIV-1 Isolation by Coculturing Macrophage With Enriched CD4+ T Cells From HIV-1 Patients
research article
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