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oai:repisalud.isciii.es:20.500.12105/152872025-06-09T15:06:47Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16961

00925njm 22002777a 4500 dc Xufré, Cristina author González, Tanía author Leal, Lorna author Trubey, Charles M author Lifson, Jeffrey D author Gatell, José María author Alcamí, José author Climent, Núria author García, Felipe author Sánchez-Palomino, Sonsoles author 2022-04 We described a novel HIV autologous isolation method based in coculturing macrophages and CD4+T-cell-enriched fractions from peripheral blood collected from antiretroviral-treated (ART) HIV patients. This method allows the isolation of high viral titers of autologous viruses, over 1010HIV RNA copies/ml, and reduces the time required to produce necessary amounts for virus for use as antigens presented by monocyte-derived myeloid cells in HIV therapeutic vaccine approaches. By applying these high titer and autologous virus produced in the patient-derived cells, we intended to elicit a boost of the immunological system response in HIV therapeutic vaccines in clinical trials. Front Virol. 2022 Apr;2:869431. 10.3389/fviro.2022.869431 2673-818X Frontiers in Virology 35967461 http://hdl.handle.net/20.500.12105/15287 CD4+ T cells HIV isolation HIV therapeutic vaccine Autologous HIV immunogen Coculture Monocyte-derived macrophages (MDM) Highly Efficient Autologous HIV-1 Isolation by Coculturing Macrophage With Enriched CD4+ T Cells From HIV-1 Patients