2026-05-15T06:24:34Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/152682024-09-21T13:56:24Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19607
Repisalud
author
Díaz-Torres, Alfonsa
author
Rosales-Nieves, Alicia E
author
Pearson, John R
author
Santa-Cruz Mateos, Carmen
author
Marín-Menguiano, Miriam
author
Marshall, Owen J
author
Brand, Andrea H
author
González-Reyes, Acaimo
funder
Agencia Estatal de Investigación (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Royal Society Darwin Trust Research Professorship
funder
Wellcome Trust
funder
European Molecular Biology Organization
2022-12-09T16:58:31Z
2022-12-09T16:58:31Z
2021-04-26
Curr Biol . 2021 Apr 26;31(8):1744-1753.e5
10.1016/j.cub.2021.01.071
1879-0445
Current biology : CB
33621481
http://hdl.handle.net/20.500.12105/15268
Stem cells reside in specialized microenvironments or niches that balance stem cell proliferation and differentiation.1,2 The extracellular matrix (ECM) is an essential component of most niches, because it controls niche homeostasis, provides physical support, and conveys extracellular signals.3-11 Basement membranes (BMs) are thin ECM sheets that are constituted mainly by Laminins, Perlecan, Collagen IV, and Entactin/Nidogen and surround epithelia and other tissues.12 Perlecans are secreted proteoglycans that interact with ECM proteins, ligands, receptors, and growth factors such as FGF, PDGF, VEGF, Hedgehog, and Wingless.13-18 Thus, Perlecans have structural and signaling functions through the binding, storage, or sequestering of specific ligands. We have used the Drosophila ovary to assess the importance of Perlecan in the functioning of a stem cell niche. Ovarioles in the adult ovary are enveloped by an ECM sheath and possess a tapered structure at their anterior apex termed the germarium. The anterior tip of the germarium hosts the germline niche, where two to four germline stem cells (GSCs) reside together with a few somatic cells: terminal filament cells (TFCs), cap cells (CpCs), and escort cells (ECs).19 We report that niche architecture in the developing gonad requires trol, that niche cells secrete an isoform-specific Perlecan-rich interstitial matrix, and that DE-cadherin-dependent stem cell-niche adhesion necessitates trol. Hence, we provide evidence to support a structural role for Perlecan in germline niche establishment during larval stages and in the maintenance of a normal pool of stem cells in the adult niche.
eng
Stem cell niche organization in the Drosophila ovary requires the ECM component Perlecan.
journal article
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URL
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Curr Biol 2021 Stem cell niche organization.pdf
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https://repisalud.isciii.es/bitstreams/90813c4a-269a-430f-b252-857e99201f38/download
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Curr Biol 2021 Stem cell niche organization.pdf.txt