2026-05-20T03:47:29Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/152672024-09-27T10:08:22Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19607

00925njm 22002777a 4500 dc Portela, Marta author Jimenez-Carretero, Daniel author Labrador, Veronica author Andreu, Maria Jose author Arza, Elvira author Caiolfa, Valeria R author Manzanares, Miguel author 2022-08-15 Most studies addressing chromatin behaviour during preimplantation development are based on biochemical assays that lack spatial and cell-specific information, crucial during early development. Here, we describe the changes in chromatin taking place at the transition from totipotency to lineage specification, by using direct stochastical optical reconstruction microscopy (dSTORM) in whole-mount embryos during the first stages of mouse development. Through the study of two post-translational modifications of Histone 3 related to active and repressed chromatin, H3K4me3 and H3K9me3 respectively, we obtained a time-course of chromatin states, showing spatial differences between cell types, related to their differentiation state. This analysis adds a new layer of information to previous biochemical studies and provides novel insight to current models of chromatin organisation during the first stages of development. Biol Open. 2022 Aug 15;11(8):bio059401 10.1242/bio.059401 2046-6390 Biology open 35876820 http://hdl.handle.net/20.500.12105/15267 Chromatin dynamics through mouse preimplantation development revealed by single molecule localisation microscopy.