2026-06-30T03:03:58Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/151762025-04-01T09:41:15Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617

00925njm 22002777a 4500 dc Garranzo-Asensio, Maria author Rodríguez-Cobos, Javier author San Millán, Coral author Poves, Carmen author Fernández-Aceñero, María Jesús author Pastor-Morate, Daniel author Viñal, David author Montero-Calle, Ana Maria author Solis-Fernandez, Guillermo author Cerón, María-Ángeles author Gámez-Chiachio, Manuel author Rodríguez, Nuria author Guzmán-Aránguez, Ana author Barderas Manchado, Rodrigo author Domínguez, Gemma author 2022-07 Colorectal cancer (CRC) is the third most common cancer and the second leading cause of cancer-related death worldwide. Alterations in proteins of the p53-family are a common event in CRC. ΔNp73, a p53-family member, shows oncogenic properties and its effectors are largely unknown. We performed an in-depth proteomics characterization of transcriptional control by ∆Np73 of the secretome of human colon cancer cells and validated its clinical potential. The secretome was analyzed using high-density antibody microarrays and stable isotopic metabolic labeling. Validation was performed by semiquantitative PCR, ELISA, dot-blot and western blot analysis. Evaluation of selected effectors was carried out using 60 plasma samples from CRC patients, individuals carrying premalignant colorectal lesions and colonoscopy-negative controls. In total, 51 dysregulated proteins were observed showing at least 1.5-foldchange in expression. We found an important association between the overexpression of ∆Np73 and effectors related to lymphangiogenesis, vasculogenesis and metastasis, such as brain-derived neurotrophic factor (BDNF) and the putative aminoacyl tRNA synthase complex-interacting multifunctional protein 1 (EMAP-II)-vascular endothelial growth factor C-vascular endothelial growth factor receptor 3 axis. We further demonstrated the usefulness of BDNF as a potential CRC biomarker able to discriminate between CRC patients and premalignant individuals from controls with high sensitivity and specificity. Mol Oncol. 2022 Jul;16(14):2672-2692. 10.1002/1878-0261.13228 1878-0261 Molecular oncology 35586989 http://hdl.handle.net/20.500.12105/15176 ΔNp73 effectors Colorectal cancer In-depthproteomics Lymphangiogenesis Secretome In-depth proteomics characterization of ∆Np73 effectors identifies key proteins with diagnostic potential implicated in lymphangiogenesis, vasculogenesis and metastasis in colorectal cancer