2026-06-14T03:37:59Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/151622024-09-27T07:58:44Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Jimenez-Carretero, Daniel
author
Ligos, José M
author
Martínez-López, María
author
Sancho, David
author
Montoya, María C
2022-11-16T08:51:45Z
2022-11-16T08:51:45Z
2018
J Immunol . 2018 May 15;200(10):3319-3331
10.4049/jimmunol.1800446
1550-6606
Journal of immunology (Baltimore, Md. : 1950)
29735643
http://hdl.handle.net/20.500.12105/15162
Advances in flow cytometry (FCM) increasingly demand adoption of computational analysis tools to tackle the ever-growing data dimensionality. In this study, we tested different data input modes to evaluate how cytometry acquisition configuration and data compensation procedures affect the performance of unsupervised phenotyping tools. An analysis workflow was set up and tested for the detection of changes in reference bead subsets and in a rare subpopulation of murine lymph node CD103+ dendritic cells acquired by conventional or spectral cytometry. Raw spectral data or pseudospectral data acquired with the full set of available detectors by conventional cytometry consistently outperformed datasets acquired and compensated according to FCM standards. Our results thus challenge the paradigm of one-fluorochrome/one-parameter acquisition in FCM for unsupervised cluster-based analysis. Instead, we propose to configure instrument acquisition to use all available fluorescence detectors and to avoid integration and compensation procedures, thereby using raw spectral or pseudospectral data for improved automated phenotypic analysis.
eng
Flow Cytometry Data Preparation Guidelines for Improved Automated Phenotypic Analysis.
journal article
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URL
https://repisalud.isciii.es/bitstreams/b0813e4e-abbd-4bc6-ab2a-2ba55a1a23f3/download
File
MD5
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J Immunol 2018 May 15 Flow Cytometry Data Preparation.pdf
URL
https://repisalud.isciii.es/bitstreams/cc4010d4-82bc-408a-8a38-bc73b70cb604/download
File
MD5
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J Immunol 2018 May 15 Flow Cytometry Data Preparation.pdf.txt