2026-07-23T17:39:44Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/151502024-09-27T22:44:24Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609col_20.500.12105_19617
Repisalud
author
Torrente-Rodríguez, Rebeca M
author
Montero-Calle, Ana Maria
author
San Bartolomé, Clara
author
Cano, Olga
author
Vazquez-Alcaraz, Monica
author
Iglesias-Caballero, Maria
author
Corral-Lugo, Andres
author
McConnell, Michael J
author
Pascal, Mariona
author
Mas-Lloret, Vicente
author
Pingarrón, José M
author
Barderas Manchado, Rodrigo
author
Campuzano, Susana
funder
Ministerio de Ciencia e Innovación (España)
funder
Comunidad de Madrid (España)
funder
Instituto de Salud Carlos III
funder
Ministerio de Educación, Cultura y Deporte (España)
2022-11-15T12:38:40Z
2022-11-15T12:38:40Z
2022-07-11
Angew Chem Int Ed Engl. 2022 Jul 11;61(28):e202203662.
10.1002/anie.202203662
1521-3773
Angewandte Chemie (International ed. in English)
35507573
http://hdl.handle.net/20.500.12105/15150
The development of versatile and sensitive biotools to quantify specific SARS-CoV-2 immunoglobulins in SARS-CoV-2 infected and non-infected individuals, built on the surface of magnetic microbeads functionalized with nucleocapsid (N) and in-house expressed recombinant spike (S) proteins is reported. Amperometric interrogation of captured N- and S-specific circulating total or individual immunoglobulin (Ig) isotypes (IgG, IgM, and IgA), subsequently labelled with HRP-conjugated secondary antibodies, was performed at disposable single or multiplexed (8×) screen-printed electrodes using the HQ/HRP/H2 O2 system. The obtained results using N and in-house expressed S ectodomains of five SARS-CoV-2 variants of concern (including the latest Delta and Omicron) allow identification of vulnerable populations from those with natural or acquired immunity, monitoring of infection, evaluation of vaccine efficiency, and even identification of the variant responsible for the infection.
eng
Electroanalytical Bioplatforms
Immune Response
Omicron
SARS-CoV-2
Viral Antigens
Towards Control and Oversight of SARS-CoV-2 Diagnosis and Monitoring through Multiplexed Quantitative Electroanalytical Immune Response Biosensors
research article
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URL
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File
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TowardsControlOversightSARS‐CoV‐2_2022.pdf
URL
https://repisalud.isciii.es/bitstreams/7de88f98-b4c5-4039-855d-b679114a9104/download
File
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text/plain
TowardsControlOversightSARS‐CoV‐2_2022.pdf.txt