2026-07-23T17:39:44Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/151502024-09-27T22:44:24Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609col_20.500.12105_19617

00925njm 22002777a 4500 dc Torrente-Rodríguez, Rebeca M author Montero-Calle, Ana Maria author San Bartolomé, Clara author Cano, Olga author Vazquez-Alcaraz, Monica author Iglesias-Caballero, Maria author Corral-Lugo, Andres author McConnell, Michael J author Pascal, Mariona author Mas-Lloret, Vicente author Pingarrón, José M author Barderas Manchado, Rodrigo author Campuzano, Susana author 2022-07-11 The development of versatile and sensitive biotools to quantify specific SARS-CoV-2 immunoglobulins in SARS-CoV-2 infected and non-infected individuals, built on the surface of magnetic microbeads functionalized with nucleocapsid (N) and in-house expressed recombinant spike (S) proteins is reported. Amperometric interrogation of captured N- and S-specific circulating total or individual immunoglobulin (Ig) isotypes (IgG, IgM, and IgA), subsequently labelled with HRP-conjugated secondary antibodies, was performed at disposable single or multiplexed (8×) screen-printed electrodes using the HQ/HRP/H2 O2 system. The obtained results using N and in-house expressed S ectodomains of five SARS-CoV-2 variants of concern (including the latest Delta and Omicron) allow identification of vulnerable populations from those with natural or acquired immunity, monitoring of infection, evaluation of vaccine efficiency, and even identification of the variant responsible for the infection. Angew Chem Int Ed Engl. 2022 Jul 11;61(28):e202203662. 10.1002/anie.202203662 1521-3773 Angewandte Chemie (International ed. in English) 35507573 http://hdl.handle.net/20.500.12105/15150 Electroanalytical Bioplatforms Immune Response Omicron SARS-CoV-2 Viral Antigens Towards Control and Oversight of SARS-CoV-2 Diagnosis and Monitoring through Multiplexed Quantitative Electroanalytical Immune Response Biosensors