2026-05-10T15:33:50Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/151262024-09-27T10:09:43Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19607
Repisalud
author
Gómez-Escolar, Carmen
author
Serrano-Navarro, Alvaro
author
Benguria, Alberto
author
Dopazo, Ana
author
Sanchez-Cabo, Fatima
author
Ramiro, Almudena R
funder
Fundación La Caixa
funder
Ministerio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España)
funder
Ministerio de Economía, Industria y Competitividad (España)
funder
Ministerio de Ciencia e Innovación (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Unión Europea. Comisión Europea. NextGenerationEU
funder
Instituto de Salud Carlos III
funder
Fundación ProCNIC
2022-11-14T12:26:30Z
2022-11-14T12:26:30Z
2022-10-07
EMBO Rep . 2022 Oct 7;e55000. doi: 10.15252/embr.202255000.
10.15252/embr.202255000
1469-3178
EMBO reports
36205653
http://hdl.handle.net/20.500.12105/15126
Germinal centers (GC) are microstructures where B cells that have been activated by antigen can improve the affinity of their B cell receptors and differentiate into memory B cells (MBCs) or antibody-secreting plasma cells. Here, we have addressed the role of activation-induced deaminase (AID), which initiates somatic hypermutation and class switch recombination, in the terminal differentiation of GC B cells. By combining single cell transcriptome and immunoglobulin clonal analysis in a mouse model that traces AID-experienced cells, we have identified a novel subset of late-prePB cells (L-prePB), which shares the strongest clonal relationships with plasmablasts (PBs). Mice lacking AID have various alterations in the size and expression profiles of transcriptional clusters. We find that AID deficiency leads to a reduced proportion of L-prePB cells and severely impairs transitions between the L-prePB and the PB subsets. Thus, AID shapes the differentiation fate of GC B cells by enabling PB generation from a prePB state.
eng
Single cell clonal analysis identifies an AID-dependent pathway of plasma cell differentiation.
journal article
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EMBO Reports _2022_Gomez_Escolar _Single cell clonal analysis identifies.pdf
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https://repisalud.isciii.es/bitstreams/ec1089a3-0e91-4f9c-a281-2d811b356778/download
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EMBO Reports _2022_Gomez_Escolar _Single cell clonal analysis identifies.pdf.txt