2026-05-10T15:33:50Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/151262024-09-27T10:09:43Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19607

00925njm 22002777a 4500 dc Gómez-Escolar, Carmen author Serrano-Navarro, Alvaro author Benguria, Alberto author Dopazo, Ana author Sanchez-Cabo, Fatima author Ramiro, Almudena R author 2022-10-07 Germinal centers (GC) are microstructures where B cells that have been activated by antigen can improve the affinity of their B cell receptors and differentiate into memory B cells (MBCs) or antibody-secreting plasma cells. Here, we have addressed the role of activation-induced deaminase (AID), which initiates somatic hypermutation and class switch recombination, in the terminal differentiation of GC B cells. By combining single cell transcriptome and immunoglobulin clonal analysis in a mouse model that traces AID-experienced cells, we have identified a novel subset of late-prePB cells (L-prePB), which shares the strongest clonal relationships with plasmablasts (PBs). Mice lacking AID have various alterations in the size and expression profiles of transcriptional clusters. We find that AID deficiency leads to a reduced proportion of L-prePB cells and severely impairs transitions between the L-prePB and the PB subsets. Thus, AID shapes the differentiation fate of GC B cells by enabling PB generation from a prePB state. EMBO Rep . 2022 Oct 7;e55000. doi: 10.15252/embr.202255000. 10.15252/embr.202255000 1469-3178 EMBO reports 36205653 http://hdl.handle.net/20.500.12105/15126 Single cell clonal analysis identifies an AID-dependent pathway of plasma cell differentiation.