2026-06-14T05:08:07Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/151122024-09-27T22:47:01Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Cañada-Garcia, Javier Enrique
author
Grippo, Natalin
author
Ramirez de Arellano, Eva
author
Bautista, Veronica
author
Lara Fuella, Noelia
author
Navarro, Ana
author
Cabezas, Teresa
author
Martínez-Ramírez, Nora Mariela
author
García-Cobos, Silvia
author
Calvo, Jorge
author
Cercenado, Emilia
author
Aracil, Belen
author
Perez-Vazquez, Maria
author
Oteo-Iglesias, Jesus
author
Spanish IMP Study Group
funder
Instituto de Salud Carlos III
funder
Centro de Investigación Biomedica en Red - CIBER
funder
Ministerio de Ciencia e Innovación (España)
funder
Unión Europea
funder
Plan Nacional de I+D+i (España)
funder
Ministerio de Economía, Industria y Competitividad (España)
funder
RETICS-Investigación en Patología Infecciosa (REIPI-ISCIII) (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
2022-11-03T10:30:38Z
2022-11-03T10:30:38Z
2022-09-28
Front Microbiol. 2022 Sep 28;13:1000787.
10.3389/fmicb.2022.1000787
1664-302X
Frontiers in microbiology
36246266
http://hdl.handle.net/20.500.12105/15112
Objectives: Little is known about IMP-producing Enterobacterales (IMP-Ent) in Europe. We analyzed at genomic and phenotypic level IMP-Ent isolates circulating in Spain in a 9-year period. Materials and methods: IMP-Ent isolates submitted to our reference laboratory were included. Antibiotic susceptibility was performed using microdilution method (EUCAST), and IMP-carbapenemase activity was measured with carbapenemase inhibitors, the β-CARBA method, the modified Hodge test (MHT), and the modified carbapenemase inhibition method (mCIM). All isolates collected were sequenced for high-resolution single-nucleotide polymorphism (SNP) typing, core genome multilocus sequence typing (cgMLST), and resistome analysis. Results: Fifty IMP-Ent isolates, collected from 19 hospitals in 13 Spanish provinces, were detected: Klebsiella pneumoniae (IMP-Kpn) (24; 48%), Enterobacter roggenkampii (13; 26%), Enterobacter hormaechei (8, 16%), Klebsiella oxytoca (two; 4%), Enterobacter asburiae (one, 2%), Serratia marcescens (one; 2%) and Escherichia coli (one; 2%). All isolates were positive by the MHT and β-CARBA tests; 48 (96%) were mCIM positive; 12 (24%) and 26 (52%) displayed positive inhibition with dipicolinic (meropenem) and EDTA (ertapenem), respectively. Five IMP-carbapenemase types were identified: IMP-8 (22; 44%), IMP-22 (17; 34%), IMP-13 (7; 14%), IMP-28 (two; 4%), and IMP-15 (two; 4%), predominating IMP-8 in K. pneumoniae and IMP-22 in E. roggenkampii. IMP-28 was exclusively identified in K. oxytoca and IMP-15 in E. hormaechei. Predominant STs were ST405 (29.2%), ST15 (25%) and ST464 (20.8%) in IMP-Kpn; ST96 (100%) in E. roggenkampii and ST182 (62.5%) in E. hormachei. Colistin and amikacin were the most active non-carbapenem antibiotics against IMP-Ent. Conclusion: IMP-Ent isolates remain infrequent in Spain, although in recent years have been circulating causing nosocomial outbreaks, being IMP-8-producing K. pneumoniae and IMP-22-producing E. roggenkampii the most frequently detected in this study. Inhibition with EDTA or dipicolinic acid presented false negative results in some IMP-producing strains. Active microbiological and molecular surveillance is essential for a better comprehension and control of IMP-Ent dissemination.
eng
Antimicrobial resistant bacteria
Carbapenem resistance
Whole genome sequencing
Surveillance
IMP carbapenemase
Enterobacterales
Phenotypic and molecular characterization of IMP-producing Enterobacterales in Spain: Predominance of IMP-8 in Klebsiella pneumoniae and IMP-22 in Enterobacter roggenkampii
journal article
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PhenotypicMolecularCharacterizationIMP-producing_2022.pdf
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Corrigendum_PhenotypicMolecularCharacterizationIMP_2023.pdf
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PhenotypicMolecularCharacterizationIMP-producing_2022.pdf.txt
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