2026-06-14T03:33:56Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/151112025-05-23T13:32:32Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Gómez González, Inmaculada
author
Perez-Vazquez, Maria
author
Tarrago Asensio, David
funder
Instituto de Salud Carlos III
2022-11-03T09:59:33Z
2022-11-03T09:59:33Z
2022-10-25
PLoS One. 2022 Oct 25;17(10):e0274058.
10.1371/journal.pone.0274058
1932-6203
PloS one
36282878
http://hdl.handle.net/20.500.12105/15111
Background: Since human herpesvirus 8 (HHV-8) infection may be underestimated and HHV-8 subtype circulation in Spain remains unknown, a molecular epidemiologic study is highly desirable. Objectives: This study aimed to analyse HHV-8 subtype diversity and their distribution in Spain. Study design: The study included 142 HHV-8 infected patients. A nested PCR was developed in order to permit Sanger sequencing of HHV-8 K1 ORF directly from clinical samples received at the CNM from 2013 to 2021. Phylogenetic characterization was performed. Results: Genotypes A and C comprised 55.6% and 42.3% of strains. Regarding subtypes, 25.4% of strains were C3, 19.7% were A3, 14.1% were A5, and C2, A1, A4, C1, A2, C7 were 11.3%, 11.3%, 8.5%, 4.2%, 2.1% and 1.4%, respectively. Subtype E1, E2 and B1 were found in only one patient each (0.7%). The Madrid region accounted for 52.1% of patients and showed a significantly different subtype distribution compared to the others (P = 0.018). Subtypes B1, E1, and E2 were observed to appear sporadically, although overall genotypes A and subtype C3 remained the most frequent and unwavering. Subtype A3 presented the highest diversity as displayed by the highest number of clusters in phylogenetic analysis. Non-significant differences in viral loads between genotypes were found, but significantly higher viral loads in subtype C2 compared to subtype C3 was found, while no significant subtype differences were observed between subtypes within genotype A. Infections with HHV-8 were detected in 94 (66.2%) patients without KS and compared to patients with KS non-significant differences in subtype distribution were found. Conclusions: Subtype prevalence and regional distribution followed a similar pattern compared to other western European countries. Our study is the first to report HHV-8 subtypes E1 and E2 circulating in Europe that might be reflective of migration of population from Caribbean countries. Our study suggests that infection by HHV-8 is underestimated, and wider screening should be recommended for risk groups.
eng
Molecular epidemiology of Kaposi sarcoma virus in Spain
research article
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URL
https://repisalud.isciii.es/bitstreams/c78616e8-e9e4-4001-84f2-786581e32ba2/download
File
MD5
399e9d561fdd258df284d22922dc7077
1243538
application/pdf
MolecularEpidemiologyKaposiSarcoma_2022.pdf
URL
https://repisalud.isciii.es/bitstreams/ea07d5a4-8a64-413e-a78e-f6aaa66d3246/download
File
MD5
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text/plain
MolecularEpidemiologyKaposiSarcoma_2022.pdf.txt