2026-04-26T23:36:59Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/150112025-05-08T15:23:44Zcom_20.500.12105_15322com_20.500.12105_2051com_20.500.12105_2052col_20.500.12105_16963col_20.500.12105_19617

00925njm 22002777a 4500 dc Montero-Calle, Ana Maria author Gómez de Cedrón, Marta author Quijada-Freire, Adriana author Solis-Fernandez, Guillermo author López-Alonso, Victoria author Espinosa-Salinas, Isabel author Peláez-García, Alberto author Fernández-Aceñero, María Jesús author Ramírez de Molina, Ana author Barderas Manchado, Rodrigo author 2022-07-25 Approximately 25% of colorectal cancer (CRC) patients experience systemic metastases, with the most frequent target organs being the liver and lung. Metabolic reprogramming has been recognized as one of the hallmarks of cancer. Here, metabolic and functional differences between two CRC cells with different metastatic organotropisms (metastatic KM12SM CRC cells to the liver and KM12L4a to the lung when injected in the spleen and in the tail vein of mice) were analysed in comparison to their parental non-metastatic isogenic KM12C cells, for a subsequent investigation of identified metabolic targets in CRC patients. Meta-analysis from proteomic and transcriptomic data deposited in databases, qPCR, WB, in vitro cell-based assays, and in vivo experiments were used to survey for metabolic alterations contributing to their different organotropism and for the subsequent analysis of identified metabolic markers in CRC patients. Although no changes in cell proliferation were observed between metastatic cells, KM12SM cells were highly dependent on oxidative phosphorylation at mitochondria, whereas KM12L4a cells were characterized by being more energetically efficient with lower basal respiration levels and a better redox management. Lipid metabolism-related targets were found altered in both cell lines, including LDLR, CD36, FABP4, SCD, AGPAT1, and FASN, which were also associated with the prognosis of CRC patients. Moreover, CD36 association with lung metastatic tropism of CRC cells was validated in vivo. Altogether, our results suggest that LDLR, CD36, FABP4, SCD, FASN, LPL, and APOA1 metabolic targets are associated with CRC metastatic tropism to the liver or lung. These features exemplify specific metabolic adaptations for invasive cancer cells which stem at the primary tumour. Front Oncol. 2022 Jul 25;12:903033. 2234-943X http://hdl.handle.net/20.500.12105/15011 35957902 10.3389/fonc.2022.903033 Frontiers in oncology CRC (colorectal cancer) Metabolic reprograming Tropism of metastasis Obesity Fatty acids (FA) Organotropism Metabolic Reprogramming Helps to Define Different Metastatic Tropisms in Colorectal Cancer