2026-05-22T17:20:56Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/149422025-05-12T11:58:04Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16963
Repisalud
author
Arca de Lafuente, Sonia
author
Casanueva-Benítez, Cristina
author
Crespo-Bermejo, Celia
author
Lara-Aguilar, Violeta
author
Martín-Carbonero, Luz
author
de Los Santos, Ignacio
author
Madrid, Ricardo
author
Briz, Veronica
funder
Comunidad de Madrid (España)
2022-09-05T07:29:43Z
2022-09-05T07:29:43Z
2022-06-16
Sci Rep. 2022 Jun 16;12(1):10124.
10.1038/s41598-022-14375-8
2045-2322
Scientific Reports
35710721
http://hdl.handle.net/20.500.12105/14942
Hepatitis C virus (HCV) infection remains a global health problem, detected only in the early stages by molecular tests. Molecular tests detect HCV RNA, which is very prone to degradation by ribonucleases, reason why blood samples must be transported and stored at − 20 °C, or even − 70 °C for long-term storage. Flinders Technology Associates (FTA) cards are a useful sampling collecting device for dry blood spot (DBS) storage, especially for low and middle-income countries (LMIC). In this study, we analyzed viral HCV RNA integrity for long-term storage at room temperature compared to − 20 °C using two diferent types of cards for DBS: FTA Classic and 903 Protein Saver cards. For this purpose, DBS were prepared on these cards using blood or plasma samples from HCV infected patients, and samples were analysed by conventional RT-PCR. Our results showed that 903 Protein Saver cards are the best and cheapest alternative for DBS storage at room temperature. In these conditions, we found that HCV RNA integrity lasted for up to 9 months.
eng
903 Protein Saver cards: the best alternative for dried blood spot storage at room temperature for HCV RNA
research article
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URL
https://repisalud.isciii.es/bitstreams/d9fee1a6-6438-4b03-b62d-f819a19d3e37/download
File
MD5
fb96d4743e7488fb865ca3be0c33e672
1654847
application/pdf
903ProteinSaverCards_2022.pdf
URL
https://repisalud.isciii.es/bitstreams/d79d98ff-560e-4c6d-8519-32e64b38ad3b/download
File
MD5
16065aedd25f6a9935f3aa5cf7230f15
37421
text/plain
903ProteinSaverCards_2022.pdf.txt