2026-06-14T03:38:22Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/148592024-10-31T11:57:46Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Topaz, Nadav
author
Tsang, Raymond
author
Deghmane, Ala-Eddine
author
Claus, Heike
author
Lâm, Thiên-Trí
author
Litt, David
author
Bajanca-Lavado, Maria Paula
author
Perez-Vazquez, Maria
author
Vestrheim, Didrik
author
Giufrè, Maria
author
Van Der Ende, Arie
author
Gaillot, Olivier
author
Kuch, Alicja
author
McElligott, Martha
author
Taha, Muhamed-Kheir
author
Wang, Xin
funder
Centers for Disease Control and Prevention (Estado Unidos)
2022-08-05T08:29:16Z
2022-08-05T08:29:16Z
2022-03-24
Front Microbiol. 2022 Mar 24;13:856884.
10.3389/fmicb.2022.856884
1664-302X
Frontiers in Microbiology
35401483
http://hdl.handle.net/20.500.12105/14859
Recent reports have indicated a rise of invasive disease caused by Haemophilus influenzae serotype a (Hia) in North America and some European countries. The whole-genome sequences for a total of 410 invasive Hia isolates were obtained from 12 countries spanning the years of 1998 to 2019 and underwent phylogenetic and comparative genomic analysis in order to characterize the major strains causing disease and the genetic variation present among factors contributing to virulence and antimicrobial resistance. Among 410 isolate sequences received, 408 passed our quality control and underwent genomic analysis. Phylogenetic analysis revealed that the Hia isolates formed four genetically distinct clades: clade 1 (n = 336), clade 2 (n = 13), clade 3 (n = 3) and clade 4 (n = 56). A low diversity subclade 1.1 was found in clade 1 and contained almost exclusively North American isolates. The predominant sequence types in the Hia collection were ST-56 (n = 125), ST-23 (n = 98) and ST-576 (n = 51), which belonged to clade 1, and ST-62 (n = 54), which belonged to clade 4. Clades 1 and 4 contained predominantly North American isolates, and clades 2 and 3 predominantly contained European isolates. Evidence of the presence of capsule duplication was detected in clade 1 and 2 isolates. Seven of the virulence genes involved in endotoxin biosynthesis were absent from all Hia isolates. In general, the presence of known factors contributing to β-lactam antibiotic resistance was low among Hia isolates. Further tests for virulence and antibiotic susceptibility would be required to determine the impact of these variations among the isolates.
eng
Haemophilus influenzae
Genomics
Invasive isolates
Phylogenetic analysis
Serotype a
Phylogenetic Structure and Comparative Genomics of Multi-National Invasive Haemophilus influenzae Serotype a Isolates
journal article
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