2026-06-14T03:36:02Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/148522025-05-12T11:57:10Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19608col_20.500.12105_16977
Repisalud
author
López-Nieva, Pilar
author
Fernandez-Navarro, Pablo L
author
Cobos-Fernández, María Ángeles
author
González-Vasconcellos, Iria
author
Sánchez Pérez, Raúl
author
Aroca, Ángel
author
Fernández-Piqueras, José
author
Santos, Javier
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Fundación Ramón Areces
funder
Comunidad de Madrid (España)
funder
Asociación Española Contra el Cáncer
funder
Banco Santander
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
2022-08-05T06:28:26Z
2022-08-05T06:28:26Z
2022-03-30
Noncoding RNA. 2022 Mar 30;8(2):26.
10.3390/ncrna8020026
2311-553X
Non-coding RNA
35447889
http://hdl.handle.net/20.500.12105/14852
Circular RNAs (circRNAs) are suggested to play a discriminative role between some stages of thymocyte differentiation. However, differential aspects of the stage of mature single-positive thymocytes remain to be explored. The purpose of this study is to investigate the differential expression pattern of circRNAs in three different development stages of human thymocytes, including mature single-positive cells, and perform predictions in silico regarding the ability of specific circRNAs when controlling the expression of genes involved in thymocyte differentiation. We isolate human thymocytes at three different stages of intrathymic differentiation and determine the expression of circRNAs and mRNA by RNASeq. We show that the differential expression pattern of 50 specific circRNAs serves to discriminate between the three human thymocyte populations. Interestingly, the downregulation of RAG2, a gene involved in T-cell differentiation in the thymus, could be simultaneously controlled by the downregulation of two circRNASs (hsa_circ_0031584 and hsa_circ_0019079) through the hypothetical liberation of hsa-miR-609. Our study provides, for the first time, significant insights into the usefulness of circRNAs in discriminating between different stages of thymocyte differentiation and provides new potential circRNA-miRNA-mRNA networks capable of controlling the expression of genes involved in T-cell differentiation in the thymus.
eng
CircRNAs
Human thymocytes
CircRNAm-iRNA-mRNA controlling networks
Patterns of Differentially Expressed circRNAs in Human Thymocytes
research article
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