2026-06-30T04:06:59Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/148512025-04-10T12:40:03Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Sempere, Julio
author
Llamosi, Mirella
author
Roman, Federico
author
Lago, Dario
author
Gonzalez-Camacho, Fernando
author
Perez-Garcia, Covadonga
author
Yuste, Jose Enrique
author
Domenech Lucas, Mirian
funder
Ministerio de Ciencia e Innovación (España)
funder
Ministerio de Economía, Industria y Competitividad (España)
funder
Ministerio de Ciencia e Innovación (España)
funder
Ministerio de Economía, Industria y Competitividad (España)
2022-08-05T06:18:44Z
2022-08-05T06:18:44Z
2022-04-23
Sci Rep. 2022 Apr 23;12(1):6668.
10.1038/s41598-022-10609-x
2045-2322
Scientific Reports
35461321
http://hdl.handle.net/20.500.12105/14851
Biofilm-associated infections are of great concern because they are associated with antibiotic resistance and immune evasion. Co-colonization by Staphylococcus aureus and Streptococcus pneumoniae is possible and a threat in clinical practice. We investigated the interaction between S. aureus and S. pneumoniae in mixed biofilms and tested new antibiofilm therapies with antioxidants N-acetyl-L-cysteine (NAC) and cysteamine (Cys). We developed two in vitro S. aureus-S. pneumoniae mixed biofilms in 96-well polystyrene microtiter plates and we treated in vitro biofilms with Cys and NAC analyzing their effect by CV staining and viable plate counting. S. pneumoniae needed a higher proportion of cells in the inoculum and planktonic culture to reach a similar population rate in the mixed biofilm. We demonstrated the effect of Cys in preventing S. aureus biofilms and S. aureus-S. pneumoniae mixed biofilms. Moreover, administration of 5 mg/ml of NAC nearly eradicated the S. pneumoniae population and killed nearly 94% of MSSA cells and 99% of MRSA cells in the mixed biofilms. The methicillin resistance background did not change the antioxidants effect in S. aureus. These results identify NAC and Cys as promising repurposed drug candidates for the prevention and treatment of mixed biofilms by S. pneumoniae and S. aureus.
eng
Clearance of mixed biofilms of Streptococcus pneumoniae and methicillin-susceptible/resistant Staphylococcus aureus by antioxidants N-acetyl-L-cysteine and cysteamine
research article
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ClearanceOfMixedBiofilmsSupplementaryFigures_2022.pdf
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ClearanceOfMixedBiofilms_2022.pdf.txt
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ClearanceOfMixedBiofilmsSupplementaryFigures_2022.pdf.txt