2026-06-14T03:38:27Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/148142025-03-21T11:02:42Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617
Repisalud
author
Anzibar Fialho, Maximiliano
author
Vázquez Alberdi, Lucia
author
Martínez, Mariana
author
Calero, Miguel
author
Baranger, Jérôme
author
Tanter, Mickael
author
Damián, Juan Pablo
author
Negreira, Carlos
author
Rubido, Nicolás
author
Kun, Alejandra
author
Brum, Javier
funder
Agencia Nacional de Investigación e Innovación (Uruguay)
2022-08-03T07:50:53Z
2022-08-03T07:50:53Z
2022
Sci Rep. 2022 Apr 26;12(1):6784.
10.1038/s41598-022-10457-9
2045-2322
Scientific Reports
35473942
http://hdl.handle.net/20.500.12105/14814
The hippocampus plays an important role in learning and memory, requiring high-neuronal oxygenation. Understanding the relationship between blood flow and vascular structure-and how it changes with ageing-is physiologically and anatomically relevant. Ultrafast Doppler ([Formula: see text]Doppler) and scanning laser confocal microscopy (SLCM) are powerful imaging modalities that can measure in vivo cerebral blood volume (CBV) and post mortem vascular structure, respectively. Here, we apply both imaging modalities to a cross-sectional and longitudinal study of hippocampi vasculature in wild-type mice brains. We introduce a segmentation of CBV distribution obtained from [Formula: see text]Doppler and show that this mice-independent and mesoscopic measurement is correlated with vessel volume fraction (VVF) distribution obtained from SLCM-e.g., high CBV relates to specific vessel locations with large VVF. Moreover, we find significant changes in CBV distribution and vasculature due to ageing (5 vs. 21 month-old mice), highlighting the sensitivity of our approach. Overall, we are able to associate CBV with vascular structure-and track its longitudinal changes-at the artery-vein, venules, arteriole, and capillary levels. We believe that this combined approach can be a powerful tool for studying other acute (e.g., brain injuries), progressive (e.g., neurodegeneration) or induced pathological changes.
eng
Ageing
Confocal microscopy
Imaging techniques
Neural ageing
Ultrasound
Intensity distribution segmentation in ultrafast Doppler combined with scanning laser confocal microscopy for assessing vascular changes associated with ageing in murine hippocampi
research article
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