2026-06-14T05:17:37Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/147572025-05-05T09:51:33Zcom_20.500.12105_14556com_20.500.12105_2337com_20.500.12105_2336com_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_14558col_20.500.12105_19617
Repisalud
author
Valverde, Alejandro
author
Montero-Calle, Ana Maria
author
Arévalo, Beatriz
author
Segundo-Acosta, Pablo San
author
Serafín, Verónica
author
Alonso‐Navarro, Miren
author
Solis-Fernandez, Guillermo
author
Pingarrón, José M
author
Campuzano, Susana
author
Barderas Manchado, Rodrigo
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Instituto de Salud Carlos III
funder
Comunidad de Madrid (España)
funder
Ministerio de Educación, Cultura y Deporte (España)
funder
Complutense University of Madrid (España)
funder
Ministerio de Economía y Competitividad (España)
funder
Research Foundation - Flanders
funder
Ministerio de Ciencia e Innovación (España)
2022-07-20T11:55:38Z
2022-07-20T11:55:38Z
2021
Analysis & Sensing. 2021;1(4):161-165
10.1002/anse.202100024
2629-2742
2629-2742
Analysis & Sensing
http://hdl.handle.net/20.500.12105/14757
An electrochemical biosensing platform for serum autoantibodies (AAbs) detection is reported in this work, exploiting for the first time six Alzheimer's disease (AD)-specific phage-derived and frameshift aberrant HaloTag peptides as receptors, immobilized on magnetic microbeads (MBs) surface and captured on disposable electrodes to perform amperometric detection. Operational analytical characteristics and clinical diagnostic ability of the bioplatform were probed in optimized key experimental conditions by analysing serum AAbs of AD patients and healthy subjects. The value of 100 % obtained for AUC, sensitivity, and selectivity from the all peptides combined ROC curve, indicate full AD-diagnostic capability of the methodology, which was further implemented, as proof of concept, in a POC multiplexing platform to detect the signature in a single test over clinically actionable times (1 h 15 min), opening great promise for the type of diagnosis and AD patients’ monitoring follow-up currently pursued.
eng
Alzheimer’sdisease
Autoantibodies
Electrochemical biosensors
Phage-derived peptides
Receptors
Phage‐Derived and Aberrant HaloTag Peptides Immobilized on Magnetic Microbeads for Amperometric Biosensing of Serum Autoantibodies and Alzheimer's Disease Diagnosis
research article
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URL
https://repisalud.isciii.es/bitstreams/b03b5962-363a-4085-b520-be6b76739175/download
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PhageDerivedAndAberrant_2021.pdf
URL
https://repisalud.isciii.es/bitstreams/e406abcc-00d6-48fc-ae0d-1c543132c79d/download
File
MD5
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text/plain
PhageDerivedAndAberrant_2021.pdf.txt