2026-06-14T03:45:58Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/147492025-03-21T10:47:26Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Köster, Pamela Carolina
author
Bailo-Barroso, Begoña
author
Dashti, Alejandro
author
Hernández-Castro, Carolina
author
Calero-Bernal, Rafael
author
Ponce-Gordo, Francisco
author
Gonzalez-Barrio, David
author
Carmena, David
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Instituto de Salud Carlos III
2022-07-20T11:02:57Z
2022-07-20T11:02:57Z
2021-05-12
Animals (Basel). 2021 May 12;11(5):1369.
10.3390/ani11051369
2076-2615
Animals : an open access journal from MDPI
34065892
http://hdl.handle.net/20.500.12105/14749
Preservation and conservation of biological specimens, including faecal samples, is a challenge in remote areas or poor-resource settings where the cold chain cannot be maintained. This study aims at evaluating the suitability of filter cards for long-term storage of faecal samples of animal and human origin positive to the diarrhoea-causing protozoan parasites, Giardia duodenalis and Cryptosporidium hominis. Three commercially available Whatman® Filter Cards were comparatively assessed: the FTA® Classic Card, the FTA® Elute Micro Card, and the 903 Protein Saver Card. Human faecal samples positive to G. duodenalis (n = 5) and C. hominis (n = 5) were used to impregnate the selected cards at given storage (1 month, 3 months, and 6 months) periods and temperature (-20 °C, 4 °C, and room temperature) conditions. Parasite DNA was detected by PCR-based methods. Sensitivity assays and quality control procedures to assess suitability for genotyping purposes were conducted. Overall, all three Whatman® cards were proven useful for the detection and molecular characterisation of G. duodenalis and C. hominis under the evaluated conditions. Whatman® cards represent a simple, safe, and cost-effective option for the transportation, preservation, and storage of faecal samples without the need of the cold chain.
eng
Cryptosporidium hominis
Giardia duodenalis
PCR
Filter card
Preservation
Storage
Transportation
Faeces
Long-Term Preservation and Storage of Faecal Samples in Whatman® Cards for PCR Detection and Genotyping of Giardia duodenalis and Cryptosporidium hominis
research article
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