2026-06-14T03:44:30Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/147262025-05-09T09:26:30Zcom_20.500.12105_15322com_20.500.12105_2051com_20.500.12105_2052col_20.500.12105_16961col_20.500.12105_16983col_20.500.12105_19617
Repisalud
author
Muñoz‐San Martín, Cristina
author
Pérez‐Ginés, Víctor
author
Torrente-Rodríguez, Rebeca M
author
Gamella, Maria
author
Solis-Fernandez, Guillermo
author
Montero-Calle, Ana Maria
author
Pedrero, María
author
Serafín, Verónica
author
Martínez‐Bosch, Neus
author
Navarro, Pilar
author
García de Frutos, Pablo
author
Batlle, Montserrat
author
Barderas Manchado, Rodrigo
author
Pingarrón, José M
author
Campuzano, Susana
funder
ECSEL Joint Undertaking
funder
Ministerio de Ciencia e Innovación (España)
funder
Comunidad de Madrid (España)
funder
Fundación La Marató TV3
funder
Instituto de Salud Carlos III
funder
Research Foundation - Flanders
funder
Complutense University of Madrid (España)
2022-07-19T07:42:40Z
2022-07-19T07:42:40Z
2021
Electrochem. Sci. Adv.2021;e2100096.
10.1002/elsa.202100096
2698-5977
2698-5977
Electrochemical Science Advances
http://hdl.handle.net/20.500.12105/14726
Growth arrest-specific 6 (GAS6) protein plays a key role in processes related toproliferation,inflammation,angiogenesis,andatheroscleroticplaqueformation.In addition, it has been reported that plasma levels of GAS6 are related to cancerprognosis and other relevant pathologies, such as heart failure or sepsis. Wereport here the first electrochemical immunoplatform for the determination ofGAS6, which has demonstrated to be competitive with other available method-ologies in terms of cost, simplicity, and decentralized application. The developedimmunoplatform involves a sandwich immunoassay using magnetic microparti-cles (MBs) and uses amperometric detection at disposable screen-printed carbonelectrodes (SPCEs). The MBs were modified with an antibody specific to GAS6for its selective capture, which is further recognized by a biotinylated secondaryantibody subsequently labeled with a streptavidin-horseradish peroxidase(Strep-HRP) conjugate. The electrochemical detection was carried out using thehydroquinone (HQ)/H2O2system. The developed bioplatform exhibits a greatselectivity and low limit of detection (27 pg/mL) that allowed the determinationof the GAS6 circulating level in plasma samples from patients suffering heartfailure (HF) and diagnosed with pancreatic ductal adenocarcinoma (PDAC),as well as the determination of the target protein in raw secretomes of humancolorectal cancer cell lines.
eng
Amperometric
GAS6
Iimmunosensor
Plasma
Secretome
Electrochemical immunosensing of Growth arrest‐specific 6 in human plasma and tumor cell secretomes
research article
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ElectrochemicaliummunosensingofGrowth_2021.pdf
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ElectrochemicaliummunosensingofGrowth_2021.pdf.txt