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00925njm 22002777a 4500 dc Muñoz‐San Martín, Cristina author Pérez‐Ginés, Víctor author Torrente-Rodríguez, Rebeca M author Gamella, Maria author Solis-Fernandez, Guillermo author Montero-Calle, Ana Maria author Pedrero, María author Serafín, Verónica author Martínez‐Bosch, Neus author Navarro, Pilar author García de Frutos, Pablo author Batlle, Montserrat author Barderas Manchado, Rodrigo author Pingarrón, José M author Campuzano, Susana author 2021 Growth arrest-specific 6 (GAS6) protein plays a key role in processes related toproliferation,inflammation,angiogenesis,andatheroscleroticplaqueformation.In addition, it has been reported that plasma levels of GAS6 are related to cancerprognosis and other relevant pathologies, such as heart failure or sepsis. Wereport here the first electrochemical immunoplatform for the determination ofGAS6, which has demonstrated to be competitive with other available method-ologies in terms of cost, simplicity, and decentralized application. The developedimmunoplatform involves a sandwich immunoassay using magnetic microparti-cles (MBs) and uses amperometric detection at disposable screen-printed carbonelectrodes (SPCEs). The MBs were modified with an antibody specific to GAS6for its selective capture, which is further recognized by a biotinylated secondaryantibody subsequently labeled with a streptavidin-horseradish peroxidase(Strep-HRP) conjugate. The electrochemical detection was carried out using thehydroquinone (HQ)/H2O2system. The developed bioplatform exhibits a greatselectivity and low limit of detection (27 pg/mL) that allowed the determinationof the GAS6 circulating level in plasma samples from patients suffering heartfailure (HF) and diagnosed with pancreatic ductal adenocarcinoma (PDAC),as well as the determination of the target protein in raw secretomes of humancolorectal cancer cell lines. Electrochem. Sci. Adv.2021;e2100096. 10.1002/elsa.202100096 2698-5977 2698-5977 Electrochemical Science Advances http://hdl.handle.net/20.500.12105/14726 Amperometric GAS6 Iimmunosensor Plasma Secretome Electrochemical immunosensing of Growth arrest‐specific 6 in human plasma and tumor cell secretomes