2026-07-23T17:11:51Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/146812024-09-27T08:02:44Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Cogliati, Sara
author
Herranz, Fernando
author
Ruiz-Cabello, Jesús
author
Enriquez, Jose Antonio
2022-07-07T11:09:20Z
2022-07-07T11:09:20Z
2021-01-01
Biochim Biophys Acta Bioenerg. 2021;1862(1):148332
10.1016/j.bbabio.2020.148332
1879-2650
Biochimica et biophysica acta. Bioenergetics
33129827
http://hdl.handle.net/20.500.12105/14681
The BlueNative page (BNGE) gel has been the reference technique for studying the electron transport chain organization since it was established 20 years ago. Although the migration of supercomplexes has been demonstrated being real, there are still several concerns about its ability to reveal genuine interactions between respiratory complexes. Moreover, the use of different solubilization conditions generates conflicting interpretations. Here, we thoroughly compare the impact of different digitonin concentrations on the liquid dispersions' physical properties and correlate with the respiratory complexes' migration pattern and supercomplexes. Our results demonstrate that digitonin concentration generates liquid dispersions with specific size and variability critical to distinguish between a real association of complexes from being trapped in the same micelle.
eng
Blue Native Electrophoresis
Respiratory Complexes
Supercomplexes
Digitonine
Mitochondria
Digitonin concentration is determinant for mitochondrial supercomplexes analysis by BlueNative page.
journal article
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1-s2.0-S0005272820301821-main.pdf.txt