2026-07-23T17:12:05Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/146812024-09-27T08:02:44Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Cogliati, Sara author Herranz, Fernando author Ruiz-Cabello, Jesús author Enriquez, Jose Antonio author 2021-01-01 The BlueNative page (BNGE) gel has been the reference technique for studying the electron transport chain organization since it was established 20 years ago. Although the migration of supercomplexes has been demonstrated being real, there are still several concerns about its ability to reveal genuine interactions between respiratory complexes. Moreover, the use of different solubilization conditions generates conflicting interpretations. Here, we thoroughly compare the impact of different digitonin concentrations on the liquid dispersions' physical properties and correlate with the respiratory complexes' migration pattern and supercomplexes. Our results demonstrate that digitonin concentration generates liquid dispersions with specific size and variability critical to distinguish between a real association of complexes from being trapped in the same micelle. Biochim Biophys Acta Bioenerg. 2021;1862(1):148332 10.1016/j.bbabio.2020.148332 1879-2650 Biochimica et biophysica acta. Bioenergetics 33129827 http://hdl.handle.net/20.500.12105/14681 Blue Native Electrophoresis Respiratory Complexes Supercomplexes Digitonine Mitochondria Digitonin concentration is determinant for mitochondrial supercomplexes analysis by BlueNative page.