2026-05-22T00:54:00Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/145382025-04-29T12:50:31Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16963

00925njm 22002777a 4500 dc Valle-Millares, Daniel author Brochado-Kith, Oscar author Gomez-Sanz, Alicia author Martín-Carbonero, Luz author Ryan, Pablo author de Los Santos, Ignacio author Castro, Juan M author Troya, Jesús author Mayoral-Muñoz, Mario author Cuevas, Guillermo author Martinez-Roman, Paula author Sanz-Sanz, Jesús author Muñoz-Muñoz, María author Jimenez-Sousa, Maria Angeles author Resino, Salvador author Briz, Veronica author Fernandez-Rodriguez, Amanda author Multidisciplinary HIV/Hepatitis Viral Coinfection Group (COVIHEP) author 2022-01 Gender-specific consequences after HCV eradication are unexplored. MicroRNAs (miRNAs) play a crucial role in the immune response against viral infections. However, few have highlighted miRNA role in sex-biased disease or therapy response. We aim to assess gender differences reflected in the miRNA expression of HIV/HCV-coinfected patients who achieve sustained virological response (SVR) with direct acting antivirals (DAAs). We conducted a prospective study of miRNA expression in PBMCs from 28 chronic HIV/HCV-coinfected patients (HIV/HCV) at baseline and after achieving SVR with DAAs. Sixteen HIV-monoinfected patients (HIV) and 36 healthy controls (HC) were used as controls. Identification of significant differentially expressed (SDE) miRNAs was performed with generalized linear model and mixed GLMs. We also explored putative dysregulated biological pathways. At baseline, the HIV/HCV patients showed differences in the miRNA profile concerning the HIV group (165 and 102 SDE miRNAs for males and females, respectively). Gender-stratified analysis of HIV/HCV group at baseline versus at SVR achievement showed higher differences in males (80 SDE miRNAs) than in females (55 SDE miRNAs). After SVR, HIV/HCV group showed similar values to HIV individuals, especially in females (1 SDE miRNA). However, ten miRNAs in males remained dysregulated, which were mainly involved in cancer, fatty acid, and inflammatory pathways. Taken together, our results show gender-biased dysregulation in the miRNA expression profile of PBMCs after HCV eradication with DAAs. These differences were normalized in females, while miRNA profile and their target-related pathways in males lack of normalization, which may be related to a high-risk of developing liver-related complications. Biomed Pharmacother. 2022 Jan;145:112405. 10.1016/j.biopha.2021.112405 1950-6007 Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie 34781145 http://hdl.handle.net/20.500.12105/14538 DAAs Gender HCV HIV High throughput sequencing MicroRNAs HCV eradication with DAAs differently affects HIV males and females: A whole miRNA sequencing characterization