2026-06-14T03:40:11Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/144822024-09-27T22:22:24Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Vélez, Nórida
author
Vega-Vela, Nelson
author
Muñoz, Marina
author
Gómez, Paola
author
Escandón, Patricia
author
Ramírez, Juan David
author
Zaragoza, Oscar
author
Monteoliva Diaz, Lucía
author
Parra-Giraldo, Claudia-Marcela
funder
Pontificia Universidad Javeriana (Colombia)
funder
Universidad del Rosario (Colombia)
funder
Ministerio de Ciencia Tecnología e Innovación (Colombia)
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Complutense University of Madrid (España)
2022-05-24T10:06:54Z
2022-05-24T10:06:54Z
2022-02-28
J Fungi (Basel). 2022 Feb 28;8(3):245.
10.3390/jof8030245
2309-608X
Journal of fungi (Basel, Switzerland)
35330247
http://hdl.handle.net/20.500.12105/14482
Background: Cryptococcus neoformans is an opportunistic fungal pathogen that can cause meningitis in immunocompromised individuals. The objective of this work was to study the relationship between the phenotypes and genotypes of isolates of clinical origin from different cities in Colombia. Methods: Genome classification of 29 clinical isolates of C. neoformans var. grubii was performed using multilocus sequence typing (MLST), and genomic sequencing was used to genotype protein-coding genes. Pathogenicity was assessed in a larval model, and melanin production and capsule size were evaluated in vitro and in vivo. Results: Eleven MLST sequence types (STs) were found, the most frequent being ST69 (n = 9), ST2, ST93, and ST377 (each with n = 4). In the 29 isolates, different levels of pigmentation, capsule size and pathogenicity were observed. Isolates classified as highly pathogenic showed a tendency to exhibit larger increases in capsule size. In the analysis of polymorphisms, 48 non-synonymous variants located in the predicted functional domains of 39 genes were found to be associated with capsule size change, melanin, or pathogenicity. Conclusions: No clear patterns were found in the analysis of the phenotype and genotype of Cryptococcus. However, the data suggest that the increase in capsule size is a key variable for the differentiation of pathogenic isolates, regardless of the method used for its induction.
eng
Cryptococcus neoformans
MLST
Capsule
Melanin
Polymorphism
Deciphering the Association among Pathogenicity, Production and Polymorphisms of Capsule/Melanin in Clinical Isolates of Cryptococcus neoformans var. grubii VNI
journal article
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DecipheringAssociationAmongPathogenicity_2022.pdf
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DecipheringAssociationAmongPathogenicitySupplementary_2022.pdf
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DecipheringAssociationAmongPathogenicity_2022.pdf.txt
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