2026-04-26T17:24:35Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/144692025-05-08T17:00:32Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16958col_20.500.12105_16982col_20.500.12105_16985
Repisalud
author
Bleriot, Ines
author
Blasco, Lucia
author
Pacios, Olga
author
Fernández-García, Laura
author
Ambroa, Antón
author
López, María
author
Ortiz-Cartagena, Concha
author
Cuenca, Felipe Fernández
author
Oteo-Iglesias, Jesus
author
Pascual, Álvaro
author
Martínez-Martínez, Luis
author
Domingo-Calap, Pilar
author
Wood, Thomas K
author
Tomás, María
funder
Instituto de Salud Carlos III
funder
Plan Nacional de I+D+i (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
RETICS-Investigación en Patología Infecciosa (REIPI-ISCIII) (España)
funder
Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica
funder
Xunta de Galicia (España)
2022-05-24T07:09:05Z
2022-05-24T07:09:05Z
2022-03-16
Sci Rep. 2022 Mar 16;12(1):4488.
http://hdl.handle.net/20.500.12105/14469
35296704
10.1038/s41598-022-08111-5
2045-2322
Scientific Reports
Since their discovery, toxin-antitoxin (TA) systems have captivated the attention of many scientists. Recent studies have demonstrated that TA systems play a key role in phage inhibition. The aim of the present study was to investigate the role of the PemIK (PemK/PemI) type II TA system in phage inhibition by its intrinsic expression in clinical strains of Klebsiella pneumoniae carrying the lncL plasmid, which harbours the carbapenemase OXA-48 and the PemK/PemI TA system. Furthermore, induced expression of the system in an IPTG-inducible plasmid in a reference strain of K. pneumoniae ATCC10031 was also studied. The results showed that induced expression of the whole TA system did not inhibit phage infection, whereas overexpression of the pemK toxin prevented early infection. To investigate the molecular mechanism involved in the PemK toxin-mediated inhibition of phage infection, assays measuring metabolic activity and viability were performed, revealing that overexpression of the PemK toxin led to dormancy of the bacteria. Thus, we demonstrate that the PemK/PemI TA system plays a role in phage infection and that the action of the free toxin induces a dormant state in the cells, resulting in inhibition of phage infections.
eng
Developmental biology
Microbiology
Molecular biology
Molecular medicine
The role of PemIK (PemK/PemI) type II TA system from Klebsiella pneumoniae clinical strains in lytic phage infection
research article
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