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oai:repisalud.isciii.es:20.500.12105/144632025-04-24T06:25:50Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Ortega, J author Infantes-Lorenzo, Jose Antonio author Roy, A author Juan Ferre, Lucia de author Romero, B author Moreno-Iruela, Inmaculada author Dominguez-Rodriguez, Mercedes author Domínguez, L author Bezos, J author 2022-07 Caprine tuberculosis (TB) is a zoonosis caused by members of the Mycobacterium tuberculosis complex (MTBC). Caprine TB eradication programmes are based mainly on intradermal tuberculin tests and slaughterhouse surveillance. However, the use of serological test has been extended as a potential diagnostic tool in goats through the use of serum, plasma, or even milk samples. Milk production and the antibodies (Ab) present in milk can vary depending on several circumstances. In the present study, different factors that may affect the performance of humoral TB diagnosis were analysed using goat milk samples: 1) lactation stage, 2) a recent previous skin test (booster effect) and 3) the effect of freeze-thaw cycles on milk samples preserved with azidiol. TB-infected animals (n = 44) were selected to evaluate the evolution of the Ab levels during the 6-month lactation period, along with its potential effect on the P22 ELISA results. In general, no significant changes (p = 0.079) were observed throughout the study as regards Ab levels in milk samples between consecutive analysis although the reactivity to P22 ELISA decreased when samplings were performed at the last two months of the lactation. Regarding the booster effect, the quantitative results showed a significant variation (p < 0.001) for both milk and serum samples when serological tests were carried out 15 days after the skin test. Finally, there were no significant differences (p = 0.99) in the P22 ELISA results when using milk samples preserved with azidiol that had undergone freeze-thaw cycles. Res Vet Sci. 2022 Jul;145:40-45. 10.1016/j.rvsc.2022.02.008 1532-2661 Research in Veterinary Science 35151157 http://hdl.handle.net/20.500.12105/14463 Azidiol Caprine tuberculosis Lactation Milk P22 ELISA Factors affecting the performance of P22 ELISA for the diagnosis of caprine tuberculosis in milk samples