2026-07-21T14:31:59Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/144492025-06-12T14:41:07Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19611col_20.500.12105_19609
Repisalud
author
Ta Tang, Thuy-Huong
author
Berzosa, Pedro
author
Rubio Gonzalez, Jose Miguel
author
Romay-Barja, Maria
author
Ncogo, Policarpo
author
Agudo, Diego
author
Herrador, Zaida
author
Cerrada-Gálvez, Laura
author
Benito, Agustin
funder
Instituto de Salud Carlos III
funder
RETICS-Investigación colaborativa en Enfermedades Tropicales (RICET-ISCIII) (España)
2022-05-23T09:42:21Z
2022-05-23T09:42:21Z
2022-02-11
Mem Inst Oswaldo Cruz. 2022 Feb 11;116:e210210.
10.1590/0074-02760210210
1678-8060
Memorias do Instituto Oswaldo Cruz
35170676
http://hdl.handle.net/20.500.12105/14449
Background: Loa loa is a filarial species found exclusively in West and Central Africa. Microscopy is the traditional diagnosis method for human loiasis. Several molecular methods have developed as an alternative approach for identification of L. loa filarial parasites. Objectives: The aim of this study was to evaluate a Loa-Loop-mediated isothermal amplification (LAMP) assay to diagnose loiasis disease on dried blood spots (DBS) samples, compared to microscopy, filaria-real time-polymerase chain reaction (PCR) and nested-Loa PCR. Methods: A total of 100 DBS samples and 100 blood smears were used for this study. DNA was extracted using saponin/Chelex method. DNA isolated was assayed by a Loa-LAMP assay in parallel to microscopy, filaria-real time PCR and nested-Loa PCR. The sensitivities and specificities of Loa-LAMP assay was computed comparing to each one of the reference methods. Findings: Loa-LAMP's sensitivity was more than 90% and specificity was nearly 100% when compared to molecular methods. On the other hand, sensitivity was decreased a bit when Loa-LAMP faced microscopy, but keeping the other statistical values high. Main conclusions: Loa-LAMP is an appropriate method for loiasis diagnosis in endemic areas. Though, it has disadvantages like the reagents' high price at the moment and not to be able to detect more filarial species at once.
eng
Filariae
Loa loa
DBS
Molecular diagnosis
LAMP
real-time PCR
Evaluation of LAMP for the diagnosis of Loa loa infection in dried blood spots compared to PCR-based assays and microscopy
research article
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EvaluationLAMPDdiagnosisLoa_2022.pdf
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text/plain
EvaluationLAMPDdiagnosisLoa_2022.pdf.txt