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oai:repisalud.isciii.es:20.500.12105/144162024-11-28T16:40:50Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16958col_20.500.12105_16982col_20.500.12105_16985

00925njm 22002777a 4500 dc Pacios, Olga author Fernández-García, Laura author Bleriot, Inés author Blasco, Lucia author Ambroa, Antón author López, María author Ortiz-Cartagena, Concha author Fernández-Cuenca, Felipe author Oteo-Iglesias, Jesus author Pascual, Álvaro author Martínez-Martínez, Luis author Domingo-Calap, Pilar author Tomás, María author 2022 Klebsiella pneumoniae is a human pathogen that worsens the prognosis of many immunocompromised patients. Here, we annotated and compared the genomes of two lytic phages that infect clinical strains of K. pneumoniae (vB_KpnM-VAC13 and vB_KpnM-VAC66) and phenotypically characterized vB_KpnM-VAC66 (time of adsorption of 12 min, burst size of 31.49 ± 0.61 PFU/infected cell, and a host range of 20.8% of the tested strains). Transmission electronic microscopy showed that vB_KpnM-VAC66 belongs to the Myoviridae family. The genomic analysis of the phage vB_KpnM-VAC66 revealed that its genome encoded 289 proteins. When compared to the genome of vB_KpnM-VAC13, they showed a nucleotide similarity of 97.56%, with a 93% of query cover, and the phylogenetic study performed with other Tevenvirinae phages showed a close common ancestor. However, there were 21 coding sequences which differed. Interestingly, the main differences were that vB_KpnM-VAC66 encoded 10 more homing endonucleases than vB_KpnM-VAC13, and that the nucleotidic and amino-acid sequences of the L-shaped tail fiber protein were highly dissimilar, leading to different three-dimensional protein predictions. Both phages differed significantly in their host range. These viruses may be useful in the development of alternative therapies to antibiotics or as a co-therapy increasing its antimicrobial potential, especially when addressing multidrug resistant (MDR) pathogens. Viruses. 2022;14(1):6. 10.3390/v14010006 1999-4915 Viruses 35062209 http://hdl.handle.net/20.500.12105/14416 Klebsiella pneumoniae L-shaped tail fiber Genomic annotation Homing endonucleases Lytic phages Phenotypic and Genomic Comparison of Klebsiella pneumoniae Lytic Phages: vB_KpnM-VAC66 and vB_KpnM-VAC13