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oai:repisalud.isciii.es:20.500.12105/143162025-04-29T12:51:18Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16938col_20.500.12105_16963

00925njm 22002777a 4500 dc Valle-Millares, Daniel author Brochado-Kith, Oscar author Martín-Carbonero, Luz author Domínguez-Domínguez, Lourdes author Ryan, Pablo author de Los Santos, Ignacio author De la Fuente, Sara author Castro, Juan M author Lagarde, María author Cuevas, Guillermo author Mayoral-Muñoz, Mario author Matarranz, Mariano author Díez, Victorino author Gomez-Sanz, Alicia author Martinez-Roman, Paula author Crespo-Bermejo, Celia author Palladino, Claudia author Muñoz-Muñoz, María author Jimenez-Sousa, Maria Angeles author Resino, Salvador author Briz, Veronica author Fernandez-Rodriguez, Amanda author Multidisciplinary HIV/Hepatitis Viral Coinfection Group (COVIHEP) author 2021-11-05 Micro RNAs (miRNAs) are essential players in HIV and HCV infections, as both viruses modulate cellular miRNAs and interact with the miRNA-mediated host response. We aim to analyze the miRNA profile of HIV patients with different exposure to HCV to explore specific signatures in the miRNA profile of PBMCs for each type of infection. We massively sequenced small RNAs of PBMCs from 117 HIV+ infected patients: 45 HIV+ patients chronically infected with HCV (HIV/HCV+), 36 HIV+ that spontaneously clarified HCV after acute infection (HIV/HCV-) and 36 HIV+ patients without previous HCV infection (HIV). Thirty-two healthy patients were used as healthy controls (HC). Differential expression analysis showed significantly differentially expressed (SDE) miRNAs in HIV/HCV+ (n = 153), HIV/HCV- (n = 169) and HIV (n = 153) patients. We found putative dysregulated pathways, such as infectious-related and PI3K signaling pathways, common in all contrasts. Specifically, putatively targeted genes involved in antifolate resistance (HIV/HV+), cancer-related pathways (HIV/HCV-) and HIF-signaling (HIV) were identified, among others. Our findings revealed that HCV strongly influences the expression profile of PBMCs from HIV patients through the disruption of its miRNome. Thus, different HCV exposure can be identified by specific miRNA signatures in PBMCs. Biomedicines. 2021 Nov 5;9(11):1627. 10.3390/biomedicines9111627 2227-9059 Biomedicines 34829855 http://hdl.handle.net/20.500.12105/14316 HCV HIV High throughput sequence MicroRNAs Spontaneous viral clearance Different HCV Exposure Drives Specific miRNA Profile in PBMCs of HIV Patients