2026-06-30T04:06:59Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/142932025-04-29T10:22:28Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16959col_20.500.12105_16963
Repisalud
author
Roman, Federico
author
Méndez-Echevarría, Ana
author
Del Rosal, Teresa
author
Garcia-Vera, Cesar
author
Escosa-Garcia, Luis
author
Agud, Martin
author
Chaves, Fernando
author
Gutiérrez-Fernández, José
author
Ruiz de Gopegui, Enrique
author
Ruiz-Carrascoso, Guillermo
author
Ruiz-Gallego, María del Carmen
author
Bernet, Albert
author
Quevedo, Sara Maria
author
Fernández-Verdugo, Ana Maria
author
Sainz, Talia
author
Calvo, Cristina
funder
Instituto de Salud Carlos III
funder
Sociedad Española Infectología Pediátrica
funder
European Society for Paediatric Infectious Diseases
funder
Asociación Española de Atención Primaria de Pediatría
2022-05-06T08:33:33Z
2022-05-06T08:33:33Z
2021-10
Microbiologyopen. 2021 Oct;10(5):e1235.
10.1002/mbo3.1235
2045-8827
MicrobiologyOpen
34713607
http://hdl.handle.net/20.500.12105/14293
Objective: To characterize the Staphylococcus aureus strains colonizing healthy Spanish children. Methods: Between March and July 2018, 1876 Spanish children younger than 14 years attending primary healthcare centers were recruited from rural and urban areas. Staphylococcus aureus colonization of the anterior nostrils was analyzed. MecA and mecC genes, antibiotic susceptibility, and genotyping according to the spa were determined in all strains, and the following toxins were examined: Panton-Valentine leucocidin (pvl), toxic shock syndrome toxin (tst), and exfoliative toxins (eta, etb, etd). Multilocus sequence typing (MLST) and staphylococcal cassette chromosome (SCCmec) typing were performed on methicillin-resistant Staphylococcus aureus (MRSA) strains, as well as pulsed-field gel electrophoresis (PFGE). Results: 619 strains were isolated in 1876 children (33%), and 92% of them were sent for characterization to the Spanish National Centre of Microbiology (n = 572). Twenty (3.5%) of these strains were mecA-positive. Several spa types were detected among MRSA, being t002 the most frequently observed (30%), associating with SCCmec IVc. Among MSSA, 33% were positive for tst, while only 0.73% were positive for pvl. The 20 MRSA strains were negative for pvl, and 6 (30%) harbored the tst gene. Conclusions: methicillin-resistant Staphylococcus aureus nasal colonization in Spanish children is rare, with t002 being the most observed spa type, associated with SCCmec IVc. None of the MRSA strains produced pvl, but up to 30% of S. aureus strains were positive for tst.
eng
Staphylococcus aureus
Methicillin-resistant
Molecular characterization
Nasal colonization
Characterization of methicillin-resistant Staphylococcus aureus strains colonizing the nostrils of Spanish children
research article
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CharacterizationOfMethicillinResistant_2021.pdf
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CharacterizationOfMethicillinResistant_2021.pdf.txt