2026-06-30T03:03:56Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/142932025-04-29T10:22:28Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16959col_20.500.12105_16963

00925njm 22002777a 4500 dc Roman, Federico author Méndez-Echevarría, Ana author Del Rosal, Teresa author Garcia-Vera, Cesar author Escosa-Garcia, Luis author Agud, Martin author Chaves, Fernando author Gutiérrez-Fernández, José author Ruiz de Gopegui, Enrique author Ruiz-Carrascoso, Guillermo author Ruiz-Gallego, María del Carmen author Bernet, Albert author Quevedo, Sara Maria author Fernández-Verdugo, Ana Maria author Sainz, Talia author Calvo, Cristina author 2021-10 Objective: To characterize the Staphylococcus aureus strains colonizing healthy Spanish children. Methods: Between March and July 2018, 1876 Spanish children younger than 14 years attending primary healthcare centers were recruited from rural and urban areas. Staphylococcus aureus colonization of the anterior nostrils was analyzed. MecA and mecC genes, antibiotic susceptibility, and genotyping according to the spa were determined in all strains, and the following toxins were examined: Panton-Valentine leucocidin (pvl), toxic shock syndrome toxin (tst), and exfoliative toxins (eta, etb, etd). Multilocus sequence typing (MLST) and staphylococcal cassette chromosome (SCCmec) typing were performed on methicillin-resistant Staphylococcus aureus (MRSA) strains, as well as pulsed-field gel electrophoresis (PFGE). Results: 619 strains were isolated in 1876 children (33%), and 92% of them were sent for characterization to the Spanish National Centre of Microbiology (n = 572). Twenty (3.5%) of these strains were mecA-positive. Several spa types were detected among MRSA, being t002 the most frequently observed (30%), associating with SCCmec IVc. Among MSSA, 33% were positive for tst, while only 0.73% were positive for pvl. The 20 MRSA strains were negative for pvl, and 6 (30%) harbored the tst gene. Conclusions: methicillin-resistant Staphylococcus aureus nasal colonization in Spanish children is rare, with t002 being the most observed spa type, associated with SCCmec IVc. None of the MRSA strains produced pvl, but up to 30% of S. aureus strains were positive for tst. Microbiologyopen. 2021 Oct;10(5):e1235. 10.1002/mbo3.1235 2045-8827 MicrobiologyOpen 34713607 http://hdl.handle.net/20.500.12105/14293 Staphylococcus aureus Methicillin-resistant Molecular characterization Nasal colonization Characterization of methicillin-resistant Staphylococcus aureus strains colonizing the nostrils of Spanish children